Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7350 | 5' | -57.7 | NC_001900.1 | + | 906 | 0.66 | 0.577394 |
Target: 5'- uCGACCAG-GCGC-UUuUCCGCGCUg -3' miRNA: 3'- cGCUGGUCuUGCGcAAcGGGCGCGAg -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 3003 | 0.68 | 0.414824 |
Target: 5'- uCGGCCucauGAACGUcgagaucaugGUUGCcaugcgCCGCGCUCg -3' miRNA: 3'- cGCUGGu---CUUGCG----------CAACG------GGCGCGAG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 4293 | 0.67 | 0.503322 |
Target: 5'- gGCGGuuccCCAGAGCGUGgggaGCCCcCGCa- -3' miRNA: 3'- -CGCU----GGUCUUGCGCaa--CGGGcGCGag -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 4720 | 0.68 | 0.405587 |
Target: 5'- cGCGACCAc-GCGCaGUggguccacgacaUGUgCCGCGCUCg -3' miRNA: 3'- -CGCUGGUcuUGCG-CA------------ACG-GGCGCGAG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 5228 | 0.69 | 0.372561 |
Target: 5'- cGCGcuggccguucccgacGCCAGaAGCgGCGUUccgguucGCUCGCGCUCa -3' miRNA: 3'- -CGC---------------UGGUC-UUG-CGCAA-------CGGGCGCGAG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 6029 | 0.75 | 0.155071 |
Target: 5'- cGCGAgCUGGAGCGaGUUGUCCGCGC-Cg -3' miRNA: 3'- -CGCU-GGUCUUGCgCAACGGGCGCGaG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 10398 | 0.72 | 0.249793 |
Target: 5'- aCGGCCAGGGCGUGau-CCCGCGUg- -3' miRNA: 3'- cGCUGGUCUUGCGCaacGGGCGCGag -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 11436 | 0.68 | 0.424189 |
Target: 5'- cCGugCGGAGCGCGg-GCaaCGCGUUCu -3' miRNA: 3'- cGCugGUCUUGCGCaaCGg-GCGCGAG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 12680 | 0.76 | 0.142861 |
Target: 5'- -gGACCAGAACG-GUcGCCCGCuGUUCa -3' miRNA: 3'- cgCUGGUCUUGCgCAaCGGGCG-CGAG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 14232 | 0.68 | 0.405587 |
Target: 5'- cGCGGCCAGGcACGuCGaUGUCCGCaaGCg- -3' miRNA: 3'- -CGCUGGUCU-UGC-GCaACGGGCG--CGag -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 17547 | 0.68 | 0.433679 |
Target: 5'- gGUGAUCuGuucauCGCGcugaUGCCCGCGCUg -3' miRNA: 3'- -CGCUGGuCuu---GCGCa---ACGGGCGCGAg -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 19095 | 0.68 | 0.443292 |
Target: 5'- gGUGACCAGGGCGUGUU-CCUG-GC-Ca -3' miRNA: 3'- -CGCUGGUCUUGCGCAAcGGGCgCGaG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 19145 | 0.7 | 0.320676 |
Target: 5'- -aGGCgCAGGAgcuuCGCGUccUGgCCGCGCUCu -3' miRNA: 3'- cgCUG-GUCUU----GCGCA--ACgGGCGCGAG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 20041 | 0.66 | 0.577394 |
Target: 5'- aGUGGCCuGAcgaGCGUUGCCCaG-GCUg -3' miRNA: 3'- -CGCUGGuCUug-CGCAACGGG-CgCGAg -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 22809 | 0.67 | 0.513678 |
Target: 5'- uGCGAUCAuGGACGCGcucagcgaagGUCCGCuGUUCc -3' miRNA: 3'- -CGCUGGU-CUUGCGCaa--------CGGGCG-CGAG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 23319 | 0.74 | 0.172818 |
Target: 5'- uGCG-CCGGGGCGCG--GCCCGCGUc- -3' miRNA: 3'- -CGCuGGUCUUGCGCaaCGGGCGCGag -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 24005 | 0.71 | 0.276471 |
Target: 5'- gGCGGCaCAGGugcuaccaGCGCGgccgaaGCCCGCGC-Ca -3' miRNA: 3'- -CGCUG-GUCU--------UGCGCaa----CGGGCGCGaG- -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 25487 | 0.66 | 0.52412 |
Target: 5'- cCGACCGuaGGCGCGUUGUCguugaCGCGCa- -3' miRNA: 3'- cGCUGGUc-UUGCGCAACGG-----GCGCGag -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 29916 | 0.71 | 0.305376 |
Target: 5'- cGCGGCCAGGACGCGaaGCUCcUGCc- -3' miRNA: 3'- -CGCUGGUCUUGCGCaaCGGGcGCGag -5' |
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7350 | 5' | -57.7 | NC_001900.1 | + | 30786 | 0.69 | 0.378668 |
Target: 5'- -aGACCcuGGACGaCGUucuUGCCCGC-CUCg -3' miRNA: 3'- cgCUGGu-CUUGC-GCA---ACGGGCGcGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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