Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7354 | 5' | -50.5 | NC_001900.1 | + | 8998 | 0.67 | 0.808954 |
|
Target: 5'- uGGCGUUCCGAGcugccaaGAAGCUCGGg---- -3' miRNA: 3'- gUCGUAAGGUUC-------CUUCGAGUCgaaga -5' |
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| 7354 | 5' | -50.5 | NC_001900.1 | + | 10626 | 0.69 | 0.723914 |
|
Target: 5'- uCGGCGUUCCAGGG--GCUCAGa---- -3' miRNA: 3'- -GUCGUAAGGUUCCuuCGAGUCgaaga -5' |
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| 7354 | 5' | -50.5 | NC_001900.1 | + | 11119 | 0.67 | 0.80996 |
|
Target: 5'- gAGUgaaUCCAuccucgAGGAAGCUCAGCgccUCa -3' miRNA: 3'- gUCGua-AGGU------UCCUUCGAGUCGa--AGa -5' |
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| 7354 | 5' | -50.5 | NC_001900.1 | + | 15125 | 0.66 | 0.872807 |
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Target: 5'- gAGCGUcggccacaccagCCGAGGcacgcuccccGAGUUCGGCUUCg -3' miRNA: 3'- gUCGUAa-----------GGUUCC----------UUCGAGUCGAAGa -5' |
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| 7354 | 5' | -50.5 | NC_001900.1 | + | 35802 | 0.67 | 0.80996 |
|
Target: 5'- cCAGUucUCC-AGG-AGCaUCGGCUUCUg -3' miRNA: 3'- -GUCGuaAGGuUCCuUCG-AGUCGAAGA- -5' |
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| 7354 | 5' | -50.5 | NC_001900.1 | + | 39974 | 1.07 | 0.002665 |
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Target: 5'- gCAGCAUUCCAAGGAAGCUCAGCUUCUc -3' miRNA: 3'- -GUCGUAAGGUUCCUUCGAGUCGAAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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