Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7356 | 3' | -57.5 | NC_001900.1 | + | 29289 | 0.66 | 0.511802 |
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Target: 5'- uGAG-CCGGCGGUUGGCGAacuccUCGUCc -3' miRNA: 3'- -UUCgGGUCGCCAGUCGCUac---AGCAGu -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 24826 | 0.66 | 0.489846 |
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Target: 5'- cGAGaCCCAGUGcugccacugggauGUCGGUGAgcgUGUUGUCAg -3' miRNA: 3'- -UUC-GGGUCGC-------------CAGUCGCU---ACAGCAGU- -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 36417 | 0.67 | 0.450299 |
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Target: 5'- -cGUUCAGgauCGGcUCGGUGAUGUCGUCc -3' miRNA: 3'- uuCGGGUC---GCC-AGUCGCUACAGCAGu -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 23331 | 0.68 | 0.393083 |
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Target: 5'- cGGCCC-GC-GUCGGCGAgaagaucGUCGUCGg -3' miRNA: 3'- uUCGGGuCGcCAGUCGCUa------CAGCAGU- -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 39879 | 0.69 | 0.374201 |
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Target: 5'- -cGCCgUGGCGGUacaucgcgugcagCAGCGGUGUCGUg- -3' miRNA: 3'- uuCGG-GUCGCCA-------------GUCGCUACAGCAgu -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 30991 | 0.7 | 0.316612 |
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Target: 5'- cAGCCCGGUGGUUu-CGGUGUCGaCGg -3' miRNA: 3'- uUCGGGUCGCCAGucGCUACAGCaGU- -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 39599 | 0.71 | 0.251903 |
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Target: 5'- uAGCCCAGCGGggCcuCGAUGUCGa-- -3' miRNA: 3'- uUCGGGUCGCCa-GucGCUACAGCagu -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 35075 | 0.71 | 0.251903 |
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Target: 5'- -cGCCuCGGCcaGGUCAGCGuaGUCGUCGu -3' miRNA: 3'- uuCGG-GUCG--CCAGUCGCuaCAGCAGU- -5' |
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| 7356 | 3' | -57.5 | NC_001900.1 | + | 40728 | 1.06 | 0.000698 |
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Target: 5'- cAAGCCCAGCGGUCAGCGAUGUCGUCAg -3' miRNA: 3'- -UUCGGGUCGCCAGUCGCUACAGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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