miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7357 3' -56.7 NC_001900.1 + 39770 0.66 0.587555
Target:  5'- aCGGGCAccguGACGcCGAuGGCCUcuGGUCGg -3'
miRNA:   3'- gGCUCGU----CUGC-GCUcUCGGAu-CCAGU- -5'
7357 3' -56.7 NC_001900.1 + 27631 0.68 0.491027
Target:  5'- uUCGAGCAGGCGUagcAGGGCuCUcgggugacguagaGGGUCGc -3'
miRNA:   3'- -GGCUCGUCUGCGc--UCUCG-GA-------------UCCAGU- -5'
7357 3' -56.7 NC_001900.1 + 38514 0.68 0.471756
Target:  5'- -gGAGCuGGCGUgggguuggGGGAGCCUGGGa-- -3'
miRNA:   3'- ggCUCGuCUGCG--------CUCUCGGAUCCagu -5'
7357 3' -56.7 NC_001900.1 + 41370 1.1 0.000532
Target:  5'- aCCGAGCAGACGCGAGAGCCUAGGUCAc -3'
miRNA:   3'- -GGCUCGUCUGCGCUCUCGGAUCCAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.