Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7357 | 5' | -63.3 | NC_001900.1 | + | 41008 | 0.66 | 0.297489 |
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Target: 5'- --gGCCUUGCcgucagGCCCCUGGCCCCa--- -3' miRNA: 3'- cugCGGGACG------CGGGGGUUGGGGgaac -5' |
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| 7357 | 5' | -63.3 | NC_001900.1 | + | 37083 | 0.66 | 0.296774 |
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Target: 5'- gGugGCCgUGCGUCgagaUCCAGCCCggagccaCCUUGu -3' miRNA: 3'- -CugCGGgACGCGG----GGGUUGGG-------GGAAC- -5' |
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| 7357 | 5' | -63.3 | NC_001900.1 | + | 8257 | 0.67 | 0.250624 |
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Target: 5'- cGACGCUCUcgaaGCGCCggCCGACaCCCCg-- -3' miRNA: 3'- -CUGCGGGA----CGCGGg-GGUUG-GGGGaac -5' |
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| 7357 | 5' | -63.3 | NC_001900.1 | + | 9917 | 0.67 | 0.22668 |
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Target: 5'- cGACuaCCUGCguaaGCCCCCGGCCgUCCa-- -3' miRNA: 3'- -CUGcgGGACG----CGGGGGUUGG-GGGaac -5' |
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| 7357 | 5' | -63.3 | NC_001900.1 | + | 10539 | 0.68 | 0.210018 |
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Target: 5'- cGACGguCCCagGCuCCCCCAACCCCa--- -3' miRNA: 3'- -CUGC--GGGa-CGcGGGGGUUGGGGgaac -5' |
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| 7357 | 5' | -63.3 | NC_001900.1 | + | 15303 | 0.68 | 0.210018 |
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Target: 5'- --gGCCCgaaCGCCUCCAcgACCCCCg-- -3' miRNA: 3'- cugCGGGac-GCGGGGGU--UGGGGGaac -5' |
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| 7357 | 5' | -63.3 | NC_001900.1 | + | 18 | 0.69 | 0.175211 |
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Target: 5'- aGGCGcCCCUGgGgggaacCCCCCuACCCCCcgUGa -3' miRNA: 3'- -CUGC-GGGACgC------GGGGGuUGGGGGa-AC- -5' |
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| 7357 | 5' | -63.3 | NC_001900.1 | + | 41404 | 1.08 | 0.000169 |
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Target: 5'- uGACGCCCUGCGCCCCCAACCCCCUUGg -3' miRNA: 3'- -CUGCGGGACGCGGGGGUUGGGGGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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