Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7358 | 5' | -49.8 | NC_001900.1 | + | 39073 | 0.66 | 0.942975 |
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Target: 5'- gGCGAGGUACGcGucgaacaGCGUcUGUCCGGu- -3' miRNA: 3'- gUGCUUCAUGCaC-------UGCA-ACAGGCUgu -5' |
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| 7358 | 5' | -49.8 | NC_001900.1 | + | 14550 | 0.67 | 0.92644 |
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Target: 5'- gGCGAugccccgAGUACaaaGUGugGUUGcUCCGAUc -3' miRNA: 3'- gUGCU-------UCAUG---CACugCAAC-AGGCUGu -5' |
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| 7358 | 5' | -49.8 | NC_001900.1 | + | 31299 | 0.69 | 0.84444 |
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Target: 5'- -cCGAAGUugauCGUGGCGUUGUCgGuGCc -3' miRNA: 3'- guGCUUCAu---GCACUGCAACAGgC-UGu -5' |
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| 7358 | 5' | -49.8 | NC_001900.1 | + | 36327 | 0.72 | 0.689082 |
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Target: 5'- -cCGcAGcGCGUccuuGACGUUGUCCGACGa -3' miRNA: 3'- guGCuUCaUGCA----CUGCAACAGGCUGU- -5' |
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| 7358 | 5' | -49.8 | NC_001900.1 | + | 43346 | 0.73 | 0.620826 |
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Target: 5'- -uCGAGGUugGUGAgcccgcCGUUGaUCCGGCGc -3' miRNA: 3'- guGCUUCAugCACU------GCAAC-AGGCUGU- -5' |
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| 7358 | 5' | -49.8 | NC_001900.1 | + | 42196 | 0.79 | 0.317679 |
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Target: 5'- uGCGGAcGUugGUGAUGUUGUCCacGACGa -3' miRNA: 3'- gUGCUU-CAugCACUGCAACAGG--CUGU- -5' |
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| 7358 | 5' | -49.8 | NC_001900.1 | + | 41521 | 1.1 | 0.003413 |
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Target: 5'- cCACGAAGUACGUGACGUUGUCCGACAg -3' miRNA: 3'- -GUGCUUCAUGCACUGCAACAGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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