Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7360 | 3' | -57.1 | NC_001900.1 | + | 24898 | 0.69 | 0.4785 |
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Target: 5'- cGGCgGGAGCCGacaucuucugggcaUCCGACguguugUCGaccaGGUGGa -3' miRNA: 3'- -CCGgCUUCGGC--------------AGGCUG------AGCg---CUACC- -5' |
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| 7360 | 3' | -57.1 | NC_001900.1 | + | 15421 | 0.7 | 0.423693 |
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Target: 5'- aGGCCGguGuCCGUCgCGACgaCGCGAUc- -3' miRNA: 3'- -CCGGCuuC-GGCAG-GCUGa-GCGCUAcc -5' |
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| 7360 | 3' | -57.1 | NC_001900.1 | + | 25758 | 0.7 | 0.396398 |
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Target: 5'- aGCCGggGCCGaCCG-C-CGUGGUGu -3' miRNA: 3'- cCGGCuuCGGCaGGCuGaGCGCUACc -5' |
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| 7360 | 3' | -57.1 | NC_001900.1 | + | 25978 | 0.71 | 0.370261 |
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Target: 5'- uGCUGGAGcCCGgaggcuacCUGGCUCGCGGUGu -3' miRNA: 3'- cCGGCUUC-GGCa-------GGCUGAGCGCUACc -5' |
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| 7360 | 3' | -57.1 | NC_001900.1 | + | 6793 | 0.71 | 0.360977 |
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Target: 5'- aGCUGAAGCUGgacgCgGACcccuacgcggacuUCGCGAUGGc -3' miRNA: 3'- cCGGCUUCGGCa---GgCUG-------------AGCGCUACC- -5' |
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| 7360 | 3' | -57.1 | NC_001900.1 | + | 47222 | 0.74 | 0.23307 |
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Target: 5'- aGGCCGAGGCCGUcacCCGGCUUucCGG-GGa -3' miRNA: 3'- -CCGGCUUCGGCA---GGCUGAGc-GCUaCC- -5' |
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| 7360 | 3' | -57.1 | NC_001900.1 | + | 16420 | 0.76 | 0.184514 |
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Target: 5'- cGCCGAAGCCagGUUCGuucGC-CGCGAUGGu -3' miRNA: 3'- cCGGCUUCGG--CAGGC---UGaGCGCUACC- -5' |
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| 7360 | 3' | -57.1 | NC_001900.1 | + | 41970 | 1.12 | 0.000427 |
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Target: 5'- gGGCCGAAGCCGUCCGACUCGCGAUGGa -3' miRNA: 3'- -CCGGCUUCGGCAGGCUGAGCGCUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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