Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7361 | 5' | -56.6 | NC_001900.1 | + | 42212 | 1.04 | 0.001165 |
Target: 5'- gUUGUCCACGACGAUCAGCGCAGGGAAg -3' miRNA: 3'- -AACAGGUGCUGCUAGUCGCGUCCCUU- -5' |
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7361 | 5' | -56.6 | NC_001900.1 | + | 23310 | 0.66 | 0.57434 |
Target: 5'- ---aCCACGGCGGUCGGCcccgGCuGGGc- -3' miRNA: 3'- aacaGGUGCUGCUAGUCG----CGuCCCuu -5' |
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7361 | 5' | -56.6 | NC_001900.1 | + | 16867 | 0.66 | 0.541291 |
Target: 5'- cUGaCCcgcacaGCGugGAUCGGCGUcGGGAu -3' miRNA: 3'- aACaGG------UGCugCUAGUCGCGuCCCUu -5' |
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7361 | 5' | -56.6 | NC_001900.1 | + | 10616 | 0.71 | 0.304549 |
Target: 5'- --aUCC-CGACGGUCGGCGUuccAGGGGc -3' miRNA: 3'- aacAGGuGCUGCUAGUCGCG---UCCCUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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