Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7362 | 5' | -60.1 | NC_001900.1 | + | 43628 | 0.66 | 0.432681 |
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Target: 5'- gGGGUGuuGCC-GAGgUcGGUucgggugucguaggcGUCCCCg -3' miRNA: 3'- gCCCACggCGGaCUCgA-CUA---------------CAGGGG- -5' |
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| 7362 | 5' | -60.1 | NC_001900.1 | + | 28172 | 0.66 | 0.410513 |
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Target: 5'- -cGGUGCCccaGCCaGAGUUguccucGAUGUCCCa -3' miRNA: 3'- gcCCACGG---CGGaCUCGA------CUACAGGGg -5' |
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| 7362 | 5' | -60.1 | NC_001900.1 | + | 36564 | 0.67 | 0.401487 |
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Target: 5'- gCGGGUGgcCCGCaugaaGAGCuuguUGAUGUCgCCu -3' miRNA: 3'- -GCCCAC--GGCGga---CUCG----ACUACAGgGG- -5' |
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| 7362 | 5' | -60.1 | NC_001900.1 | + | 11950 | 0.67 | 0.383819 |
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Target: 5'- -cGGUGCUGCCUGAGUUcuacaaGGUGgUCCg- -3' miRNA: 3'- gcCCACGGCGGACUCGA------CUAC-AGGgg -5' |
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| 7362 | 5' | -60.1 | NC_001900.1 | + | 24392 | 0.68 | 0.318474 |
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Target: 5'- aCGGGaUGUcgacgccaCGCCUGAGCgugGccGUCaCCCa -3' miRNA: 3'- -GCCC-ACG--------GCGGACUCGa--CuaCAG-GGG- -5' |
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| 7362 | 5' | -60.1 | NC_001900.1 | + | 6763 | 0.75 | 0.118858 |
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Target: 5'- aGGGUGUCGCC-GAGCUGA--UCCUg -3' miRNA: 3'- gCCCACGGCGGaCUCGACUacAGGGg -5' |
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| 7362 | 5' | -60.1 | NC_001900.1 | + | 43147 | 1.1 | 0.000253 |
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Target: 5'- uCGGGUGCCGCCUGAGCUGAUGUCCCCc -3' miRNA: 3'- -GCCCACGGCGGACUCGACUACAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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