Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7363 | 3' | -57.2 | NC_001900.1 | + | 4075 | 0.66 | 0.586892 |
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Target: 5'- gACG-UGCUCGCaCgUGGCUUCCggCAUGa -3' miRNA: 3'- -UGCaACGAGUGcG-ACCGAGGGa-GUGC- -5' |
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| 7363 | 3' | -57.2 | NC_001900.1 | + | 41078 | 0.66 | 0.565129 |
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Target: 5'- gACGUUGCgggcCAgGCcGGUgaCCUCGCGg -3' miRNA: 3'- -UGCAACGa---GUgCGaCCGagGGAGUGC- -5' |
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| 7363 | 3' | -57.2 | NC_001900.1 | + | 23808 | 0.67 | 0.479787 |
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Target: 5'- gACGacggGCUCcggucggcuaucgGCGCUGGCUUCCggcgCugGg -3' miRNA: 3'- -UGCaa--CGAG-------------UGCGACCGAGGGa---GugC- -5' |
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| 7363 | 3' | -57.2 | NC_001900.1 | + | 42904 | 0.68 | 0.450755 |
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Target: 5'- aGCGUUGCUCGCGgUGuGgUCgCCgacgUACGg -3' miRNA: 3'- -UGCAACGAGUGCgAC-CgAG-GGa---GUGC- -5' |
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| 7363 | 3' | -57.2 | NC_001900.1 | + | 46458 | 0.68 | 0.44097 |
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Target: 5'- gGCGUUGUUCugGUc-GCUUCCUC-CGg -3' miRNA: 3'- -UGCAACGAGugCGacCGAGGGAGuGC- -5' |
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| 7363 | 3' | -57.2 | NC_001900.1 | + | 43309 | 1.08 | 0.000634 |
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Target: 5'- gACGUUGCUCACGCUGGCUCCCUCACGu -3' miRNA: 3'- -UGCAACGAGUGCGACCGAGGGAGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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