Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7365 | 3' | -55.4 | NC_001900.1 | + | 30596 | 0.66 | 0.686754 |
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Target: 5'- -cUCGAugacgGGCUG-GAacCCUUCCUCGAa -3' miRNA: 3'- guAGCU-----CCGACgCUacGGAAGGAGCU- -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 11224 | 0.66 | 0.635725 |
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Target: 5'- aCAUCGAGGaguggcacgcagggUGCGAcUGCCUgguggugccugUCUUCGAc -3' miRNA: 3'- -GUAGCUCCg-------------ACGCU-ACGGA-----------AGGAGCU- -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 25202 | 0.67 | 0.586826 |
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Target: 5'- --cCGAGGUUGCGGUuacgggccugGCCUUCC-Ca- -3' miRNA: 3'- guaGCUCCGACGCUA----------CGGAAGGaGcu -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 14296 | 0.68 | 0.553867 |
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Target: 5'- -uUCGAGGacgGCGGUGCgCUgaccggUCUCGAu -3' miRNA: 3'- guAGCUCCga-CGCUACG-GAa-----GGAGCU- -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 48193 | 0.68 | 0.532198 |
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Target: 5'- gGUCG-GGCUGCGGc-CCUUCC-CGGu -3' miRNA: 3'- gUAGCuCCGACGCUacGGAAGGaGCU- -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 13440 | 0.68 | 0.510842 |
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Target: 5'- aCAUCGAGGCcgacGCGGUGCUgcgUCUggugCGu -3' miRNA: 3'- -GUAGCUCCGa---CGCUACGGa--AGGa---GCu -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 25935 | 0.7 | 0.439259 |
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Target: 5'- aCGUCGc-GCUcaGCGGUGCCUUCUgcUCGAu -3' miRNA: 3'- -GUAGCucCGA--CGCUACGGAAGG--AGCU- -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 36646 | 0.72 | 0.307418 |
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Target: 5'- gGUCGAGGUUGCgGGUGaUCU-CCUCGGc -3' miRNA: 3'- gUAGCUCCGACG-CUAC-GGAaGGAGCU- -5' |
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| 7365 | 3' | -55.4 | NC_001900.1 | + | 44088 | 1.09 | 0.000835 |
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Target: 5'- aCAUCGAGGCUGCGAUGCCUUCCUCGAc -3' miRNA: 3'- -GUAGCUCCGACGCUACGGAAGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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