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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 737 | 3' | -53.8 | AC_000020.1 | + | 14280 | 0.66 | 0.603758 |
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Target: 5'- -gUGGCgCGCAacgGUCCCccggcGGCgcgcuuggugcuggaGGCUGCCCg -3' miRNA: 3'- agAUCG-GUGU---UAGGG-----UCG---------------UCGAUGGG- -5' |
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| 737 | 3' | -53.8 | AC_000020.1 | + | 15420 | 0.66 | 0.599094 |
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Target: 5'- cCU-GCCAC---CCCAGCuaccGCUGCCg -3' miRNA: 3'- aGAuCGGUGuuaGGGUCGu---CGAUGGg -5' |
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| 737 | 3' | -53.8 | AC_000020.1 | + | 25007 | 0.66 | 0.587458 |
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Target: 5'- aCUGGUuuUACAA-CCCAGCA-CUugCCu -3' miRNA: 3'- aGAUCG--GUGUUaGGGUCGUcGAugGG- -5' |
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| 737 | 3' | -53.8 | AC_000020.1 | + | 25752 | 0.66 | 0.587458 |
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Target: 5'- gCUGGUauaaaguaGAcCCCAGCGGCUuccaaGCCCa -3' miRNA: 3'- aGAUCGgug-----UUaGGGUCGUCGA-----UGGG- -5' |
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| 737 | 3' | -53.8 | AC_000020.1 | + | 17349 | 0.67 | 0.541435 |
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Target: 5'- cUUUAGCC-CAGgugCCCucuGCAGgUGCCa -3' miRNA: 3'- -AGAUCGGuGUUa--GGGu--CGUCgAUGGg -5' |
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| 737 | 3' | -53.8 | AC_000020.1 | + | 5151 | 0.68 | 0.453662 |
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Target: 5'- gCUAGUCuuuGAUCCCAGCA---ACCCa -3' miRNA: 3'- aGAUCGGug-UUAGGGUCGUcgaUGGG- -5' |
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| 737 | 3' | -53.8 | AC_000020.1 | + | 10778 | 0.7 | 0.393168 |
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Target: 5'- cUCUAGCUccgGCAGUCCguuagaAGCcaaGGCUugCCg -3' miRNA: 3'- -AGAUCGG---UGUUAGGg-----UCG---UCGAugGG- -5' |
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| 737 | 3' | -53.8 | AC_000020.1 | + | 13667 | 1.13 | 0.000284 |
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Target: 5'- cUCUAGCCACAAUCCCAGCAGCUACCCa -3' miRNA: 3'- -AGAUCGGUGUUAGGGUCGUCGAUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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