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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 737 | 5' | -53.1 | AC_000020.1 | + | 29728 | 0.66 | 0.62611 |
|
Target: 5'- aGCugGGuGgUGCaUACCAcaUAgUGCCAg -3' miRNA: 3'- -CGugCCuCgACG-AUGGUa-AUgACGGU- -5' |
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| 737 | 5' | -53.1 | AC_000020.1 | + | 25591 | 0.71 | 0.339163 |
|
Target: 5'- uUAUGGAGaCUGCUACCAa-ACUGCa- -3' miRNA: 3'- cGUGCCUC-GACGAUGGUaaUGACGgu -5' |
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| 737 | 5' | -53.1 | AC_000020.1 | + | 17527 | 0.67 | 0.572155 |
|
Target: 5'- gGCACGGccagacgcaucccccAGCU-CUGCUAg-GCUGCCAu -3' miRNA: 3'- -CGUGCC---------------UCGAcGAUGGUaaUGACGGU- -5' |
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| 737 | 5' | -53.1 | AC_000020.1 | + | 16065 | 0.69 | 0.430734 |
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Target: 5'- aGCuuGGAGC-GCUACCGaagcuauuccuggUACUGCCc -3' miRNA: 3'- -CGugCCUCGaCGAUGGUa------------AUGACGGu -5' |
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| 737 | 5' | -53.1 | AC_000020.1 | + | 15639 | 0.73 | 0.281819 |
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Target: 5'- aGCAUGGuGuCUGCggugACCAgaGCUGCCc -3' miRNA: 3'- -CGUGCCuC-GACGa---UGGUaaUGACGGu -5' |
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| 737 | 5' | -53.1 | AC_000020.1 | + | 13701 | 1.11 | 0.00048 |
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Target: 5'- gGCACGGAGCUGCUACCAUUACUGCCAa -3' miRNA: 3'- -CGUGCCUCGACGAUGGUAAUGACGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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