Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 738 | 3' | -56.4 | AC_000020.1 | + | 15604 | 0.65 | 0.499493 |
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Target: 5'- gGCuuGGGGCCCCUCauuuuccuagccgUGAUgCUGGggUg -3' miRNA: 3'- gCG--CCCCGGGGAGa------------ACUGgGAUUuuG- -5' |
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| 738 | 3' | -56.4 | AC_000020.1 | + | 23815 | 0.67 | 0.430942 |
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Target: 5'- uGCGGGGCUgCUU---ACCCaGAGGCu -3' miRNA: 3'- gCGCCCCGGgGAGaacUGGGaUUUUG- -5' |
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| 738 | 3' | -56.4 | AC_000020.1 | + | 12455 | 0.67 | 0.411274 |
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Target: 5'- aGCGGGGCCUguaCUgCUUaGGCCUUAcuACc -3' miRNA: 3'- gCGCCCCGGG---GA-GAA-CUGGGAUuuUG- -5' |
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| 738 | 3' | -56.4 | AC_000020.1 | + | 20859 | 0.67 | 0.411274 |
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Target: 5'- aGCGGGGCCUCUUgcgguaugUuuuauaccgaugGACCC-AAAGCu -3' miRNA: 3'- gCGCCCCGGGGAGa-------A------------CUGGGaUUUUG- -5' |
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| 738 | 3' | -56.4 | AC_000020.1 | + | 14265 | 0.68 | 0.338447 |
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Target: 5'- --aGGGGCCCCgcgggCUcGGCCUUGAcGGCg -3' miRNA: 3'- gcgCCCCGGGGa----GAaCUGGGAUU-UUG- -5' |
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| 738 | 3' | -56.4 | AC_000020.1 | + | 14222 | 1.09 | 0.000315 |
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Target: 5'- cCGCGGGGCCCCUCUUGACCCUAAAACu -3' miRNA: 3'- -GCGCCCCGGGGAGAACUGGGAUUUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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