Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 739 | 3' | -57.8 | AC_000020.1 | + | 16405 | 0.66 | 0.401834 |
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Target: 5'- --uCAAGUGCCCcgUGCaCCCCgGuGGAa -3' miRNA: 3'- aguGUUCACGGGuaGUG-GGGGgU-CCU- -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 21422 | 0.66 | 0.392425 |
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Target: 5'- gUUugGAGcaaGCCCAaCGCgCCCUCGGGGc -3' miRNA: 3'- -AGugUUCa--CGGGUaGUG-GGGGGUCCU- -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 24288 | 0.66 | 0.374044 |
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Target: 5'- aCACGcc-GCCgG-CGCCUCCCAGGAc -3' miRNA: 3'- aGUGUucaCGGgUaGUGGGGGGUCCU- -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 1258 | 0.67 | 0.365075 |
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Target: 5'- cUCACAGGgaguagcugcUGaCCUcuuUCugCCCCCAGGu -3' miRNA: 3'- -AGUGUUC----------AC-GGGu--AGugGGGGGUCCu -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 17278 | 0.67 | 0.33907 |
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Target: 5'- cUACAA-UGCCC-UCGCCCCcaaggCCGGGGc -3' miRNA: 3'- aGUGUUcACGGGuAGUGGGG-----GGUCCU- -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 24823 | 0.67 | 0.321681 |
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Target: 5'- gCACAaacugguaGGUGCCCA-CGCCUCCUgcgcgcggcgucgAGGAu -3' miRNA: 3'- aGUGU--------UCACGGGUaGUGGGGGG-------------UCCU- -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 19550 | 0.68 | 0.314436 |
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Target: 5'- -gGCAcGcGUCCAUCAgCCCCaCCGGGGg -3' miRNA: 3'- agUGUuCaCGGGUAGU-GGGG-GGUCCU- -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 2714 | 0.71 | 0.17432 |
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Target: 5'- -gGCAauAGUGCCC-UgACCCCCCAGc- -3' miRNA: 3'- agUGU--UCACGGGuAgUGGGGGGUCcu -5' |
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| 739 | 3' | -57.8 | AC_000020.1 | + | 14958 | 1.01 | 0.001076 |
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Target: 5'- cUCACAAGUGCCC-UCACCCCCCAGGAg -3' miRNA: 3'- -AGUGUUCACGGGuAGUGGGGGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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