miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
740 5' -55.3 AC_000020.1 + 21137 0.69 0.364626
Target:  5'- -aAGCAGCAgagCUUGCCccUGGGGUuaaccccaGCGGg -3'
miRNA:   3'- ggUUGUCGUa--GGACGG--ACUCCA--------CGCC- -5'
740 5' -55.3 AC_000020.1 + 14597 0.74 0.193549
Target:  5'- uCCAGCAGU-UCCUGCUUGAuGGcGCGa -3'
miRNA:   3'- -GGUUGUCGuAGGACGGACU-CCaCGCc -5'
740 5' -55.3 AC_000020.1 + 15627 0.74 0.183026
Target:  5'- cCCAGCGGCugAUCUUGCCUGuAGGcuUGgGGc -3'
miRNA:   3'- -GGUUGUCG--UAGGACGGAC-UCC--ACgCC- -5'
740 5' -55.3 AC_000020.1 + 15603 1.11 0.000325
Target:  5'- gCCAACAGCAUCCUGCCUGAGGUGCGGc -3'
miRNA:   3'- -GGUUGUCGUAGGACGGACUCCACGCC- -5'
740 5' -55.3 AC_000020.1 + 24738 0.69 0.401652
Target:  5'- gCAACAGgCAUCCUcgacGCCgcgcgcagGAGGcGUGGg -3'
miRNA:   3'- gGUUGUC-GUAGGA----CGGa-------CUCCaCGCC- -5'
740 5' -55.3 AC_000020.1 + 24667 0.66 0.570209
Target:  5'- cCCGACGGCAuauUCCaGCUagGAGG-GgGGu -3'
miRNA:   3'- -GGUUGUCGU---AGGaCGGa-CUCCaCgCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.