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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 740 | 5' | -55.3 | AC_000020.1 | + | 21137 | 0.69 | 0.364626 |
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Target: 5'- -aAGCAGCAgagCUUGCCccUGGGGUuaaccccaGCGGg -3' miRNA: 3'- ggUUGUCGUa--GGACGG--ACUCCA--------CGCC- -5' |
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| 740 | 5' | -55.3 | AC_000020.1 | + | 14597 | 0.74 | 0.193549 |
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Target: 5'- uCCAGCAGU-UCCUGCUUGAuGGcGCGa -3' miRNA: 3'- -GGUUGUCGuAGGACGGACU-CCaCGCc -5' |
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| 740 | 5' | -55.3 | AC_000020.1 | + | 15627 | 0.74 | 0.183026 |
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Target: 5'- cCCAGCGGCugAUCUUGCCUGuAGGcuUGgGGc -3' miRNA: 3'- -GGUUGUCG--UAGGACGGAC-UCC--ACgCC- -5' |
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| 740 | 5' | -55.3 | AC_000020.1 | + | 15603 | 1.11 | 0.000325 |
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Target: 5'- gCCAACAGCAUCCUGCCUGAGGUGCGGc -3' miRNA: 3'- -GGUUGUCGUAGGACGGACUCCACGCC- -5' |
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| 740 | 5' | -55.3 | AC_000020.1 | + | 24738 | 0.69 | 0.401652 |
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Target: 5'- gCAACAGgCAUCCUcgacGCCgcgcgcagGAGGcGUGGg -3' miRNA: 3'- gGUUGUC-GUAGGA----CGGa-------CUCCaCGCC- -5' |
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| 740 | 5' | -55.3 | AC_000020.1 | + | 24667 | 0.66 | 0.570209 |
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Target: 5'- cCCGACGGCAuauUCCaGCUagGAGG-GgGGu -3' miRNA: 3'- -GGUUGUCGU---AGGaCGGa-CUCCaCgCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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