Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 742 | 5' | -60 | AC_000020.1 | + | 648 | 0.66 | 0.322395 |
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Target: 5'- gGCGgcGAGGGGCugCCAugagaaUACUCAcagucaagGCAg -3' miRNA: 3'- gCGCa-CUCCCCGugGGU------AUGGGU--------CGU- -5' |
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| 742 | 5' | -60 | AC_000020.1 | + | 22422 | 0.66 | 0.314455 |
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Target: 5'- gGCGcuGGGGGCgGCCCuuuUAgCUAGCAg -3' miRNA: 3'- gCGCacUCCCCG-UGGGu--AUgGGUCGU- -5' |
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| 742 | 5' | -60 | AC_000020.1 | + | 15558 | 0.67 | 0.276981 |
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Target: 5'- ---aUGAGGGGC-CCCAaGCCUacaGGCAa -3' miRNA: 3'- gcgcACUCCCCGuGGGUaUGGG---UCGU- -5' |
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| 742 | 5' | -60 | AC_000020.1 | + | 27323 | 0.68 | 0.241242 |
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Target: 5'- gGCGgggGAGGGGUugCCAccaaagcuccgcucUGCaCGGCAa -3' miRNA: 3'- gCGCa--CUCCCCGugGGU--------------AUGgGUCGU- -5' |
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| 742 | 5' | -60 | AC_000020.1 | + | 19281 | 1.08 | 0.000163 |
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Target: 5'- cCGCGUGAGGGGCACCCAUACCCAGCAa -3' miRNA: 3'- -GCGCACUCCCCGUGGGUAUGGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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