miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
742 5' -60 AC_000020.1 + 19281 1.08 0.000163
Target:  5'- cCGCGUGAGGGGCACCCAUACCCAGCAa -3'
miRNA:   3'- -GCGCACUCCCCGUGGGUAUGGGUCGU- -5'
742 5' -60 AC_000020.1 + 27323 0.68 0.241242
Target:  5'- gGCGgggGAGGGGUugCCAccaaagcuccgcucUGCaCGGCAa -3'
miRNA:   3'- gCGCa--CUCCCCGugGGU--------------AUGgGUCGU- -5'
742 5' -60 AC_000020.1 + 15558 0.67 0.276981
Target:  5'- ---aUGAGGGGC-CCCAaGCCUacaGGCAa -3'
miRNA:   3'- gcgcACUCCCCGuGGGUaUGGG---UCGU- -5'
742 5' -60 AC_000020.1 + 648 0.66 0.322395
Target:  5'- gGCGgcGAGGGGCugCCAugagaaUACUCAcagucaagGCAg -3'
miRNA:   3'- gCGCa-CUCCCCGugGGU------AUGGGU--------CGU- -5'
742 5' -60 AC_000020.1 + 22422 0.66 0.314455
Target:  5'- gGCGcuGGGGGCgGCCCuuuUAgCUAGCAg -3'
miRNA:   3'- gCGCacUCCCCG-UGGGu--AUgGGUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.