Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
743 | 3' | -53.1 | AC_000020.1 | + | 10308 | 0.66 | 0.643886 |
Target: 5'- aCUGC-CCAgcucuuucuuaUAGUGcAACACUGCAGGg- -3' miRNA: 3'- -GACGcGGU-----------AUCAU-UUGUGGCGUCCgg -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 15917 | 0.66 | 0.643886 |
Target: 5'- -gGCGCCcacgcGGcauACGCUGCGGGCg -3' miRNA: 3'- gaCGCGGua---UCauuUGUGGCGUCCGg -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 27534 | 0.66 | 0.643886 |
Target: 5'- -gGgGCUGUAGUAGG---UGCAGGCCu -3' miRNA: 3'- gaCgCGGUAUCAUUUgugGCGUCCGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 10983 | 0.66 | 0.643886 |
Target: 5'- aCUGuCGUCGgcuGUAGGCugacuCUGCAuGGCCg -3' miRNA: 3'- -GAC-GCGGUau-CAUUUGu----GGCGU-CCGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 4806 | 0.66 | 0.63218 |
Target: 5'- gUGgGCCugcacgcUGGUGAGggucCugCGCAGGCUc -3' miRNA: 3'- gACgCGGu------AUCAUUU----GugGCGUCCGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 15358 | 0.66 | 0.631009 |
Target: 5'- -aGCGCC-------GCACCGCAGcuGCCa -3' miRNA: 3'- gaCGCGGuaucauuUGUGGCGUC--CGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 14800 | 0.67 | 0.620474 |
Target: 5'- -cGCGCCGaccccgccacUGGUAggGACGCCGgGGaGCa -3' miRNA: 3'- gaCGCGGU----------AUCAU--UUGUGGCgUC-CGg -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 16744 | 0.67 | 0.573876 |
Target: 5'- -gGCGCCGggggggAGUAGGCugGCgGCuGGUCa -3' miRNA: 3'- gaCGCGGUa-----UCAUUUG--UGgCGuCCGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 7937 | 0.68 | 0.516901 |
Target: 5'- -gGCGCCGUAGUGGu--CCaGCGcGCCa -3' miRNA: 3'- gaCGCGGUAUCAUUuguGG-CGUcCGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 17631 | 0.69 | 0.499133 |
Target: 5'- --aUGCCAUGcuauacacugaagauGUAAAC-CUGCAGGCCc -3' miRNA: 3'- gacGCGGUAU---------------CAUUUGuGGCGUCCGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 17725 | 0.69 | 0.494733 |
Target: 5'- gCUGgGCCAgcAGgcuGCGCCcaaCAGGCCg -3' miRNA: 3'- -GACgCGGUa-UCauuUGUGGc--GUCCGG- -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 21575 | 0.71 | 0.345268 |
Target: 5'- -gGCGCUucuuUGG-GGGCGCCGUAGGCg -3' miRNA: 3'- gaCGCGGu---AUCaUUUGUGGCGUCCGg -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 14851 | 0.72 | 0.303176 |
Target: 5'- uCUG-GCCGggAGUAAACACCaCGGGCa -3' miRNA: 3'- -GACgCGGUa-UCAUUUGUGGcGUCCGg -5' |
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743 | 3' | -53.1 | AC_000020.1 | + | 19765 | 1.13 | 0.000362 |
Target: 5'- cCUGCGCCAUAGUAAACACCGCAGGCCg -3' miRNA: 3'- -GACGCGGUAUCAUUUGUGGCGUCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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