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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 7436 | 5' | -41.9 | NC_001902.1 | + | 23047 | 0.66 | 0.998795 |
|
Target: 5'- -cUCAGGcacgGGUGGuUCAGGggGAGu- -3' miRNA: 3'- gaAGUCUa---UUACUuAGUCCuuCUUcc -5' |
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| 7436 | 5' | -41.9 | NC_001902.1 | + | 22801 | 0.66 | 0.998795 |
|
Target: 5'- -cUCAGGcacgGGUGGuUCAGGggGAGu- -3' miRNA: 3'- gaAGUCUa---UUACUuAGUCCuuCUUcc -5' |
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| 7436 | 5' | -41.9 | NC_001902.1 | + | 2049 | 0.67 | 0.997116 |
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Target: 5'- -gUCAGAguacGAUGAGgaugaaAGGAAGAguaAGGa -3' miRNA: 3'- gaAGUCUa---UUACUUag----UCCUUCU---UCC- -5' |
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| 7436 | 5' | -41.9 | NC_001902.1 | + | 999 | 0.67 | 0.997116 |
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Target: 5'- cCUUCAuc--GUGAgGUCAGGGAGuGGGg -3' miRNA: 3'- -GAAGUcuauUACU-UAGUCCUUCuUCC- -5' |
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| 7436 | 5' | -41.9 | NC_001902.1 | + | 18953 | 0.7 | 0.975821 |
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Target: 5'- --cCGGGU-AUGAucuuGUCAGGGAGgcGGg -3' miRNA: 3'- gaaGUCUAuUACU----UAGUCCUUCuuCC- -5' |
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| 7436 | 5' | -41.9 | NC_001902.1 | + | 21703 | 1.15 | 0.005582 |
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Target: 5'- cCUUCAGAUAAUGAAUCAGGAAGAAGGa -3' miRNA: 3'- -GAAGUCUAUUACUUAGUCCUUCUUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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