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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7438 | 5' | -42.9 | NC_001902.1 | + | 25016 | 1.15 | 0.003951 |
|
Target: 5'- cCAAAAGAACUUCCUUCUAAAACCCCCu -3' miRNA: 3'- -GUUUUCUUGAAGGAAGAUUUUGGGGG- -5' |
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| 7438 | 5' | -42.9 | NC_001902.1 | + | 24973 | 0.66 | 0.995643 |
|
Target: 5'- aGAAGGAAgUUCUUUUggc-GCCCaCCa -3' miRNA: 3'- gUUUUCUUgAAGGAAGauuuUGGG-GG- -5' |
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| 7438 | 5' | -42.9 | NC_001902.1 | + | 22015 | 0.66 | 0.997048 |
|
Target: 5'- ----cGAGCUUCCaag-GAAAUCCCUc -3' miRNA: 3'- guuuuCUUGAAGGaagaUUUUGGGGG- -5' |
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| 7438 | 5' | -42.9 | NC_001902.1 | + | 19014 | 0.66 | 0.995643 |
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Target: 5'- --cAGGAACagCCUUCcccgGAuACCCCg -3' miRNA: 3'- guuUUCUUGaaGGAAGa---UUuUGGGGg -5' |
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| 7438 | 5' | -42.9 | NC_001902.1 | + | 15070 | 0.79 | 0.531298 |
|
Target: 5'- uGAAAGAACUUCUUagUGAAAUCCCa -3' miRNA: 3'- gUUUUCUUGAAGGAagAUUUUGGGGg -5' |
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| 7438 | 5' | -42.9 | NC_001902.1 | + | 3528 | 0.68 | 0.978662 |
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Target: 5'- -cAAGGAACUUCacagaAAAGCCUCCa -3' miRNA: 3'- guUUUCUUGAAGgaagaUUUUGGGGG- -5' |
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| 7438 | 5' | -42.9 | NC_001902.1 | + | 2170 | 0.67 | 0.992557 |
|
Target: 5'- ---uGGuGCUUCacgUUUAAGACCCCUu -3' miRNA: 3'- guuuUCuUGAAGga-AGAUUUUGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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