Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7439 | 3' | -48.5 | NC_001902.1 | + | 21705 | 0.68 | 0.808874 |
Target: 5'- aGGGGUACCUUCGaggcgccucaCUGAcaauggcaUGUACcguGUACAg -3' miRNA: 3'- -CCCUAUGGAAGC----------GGCU--------ACAUG---UAUGU- -5' |
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7439 | 3' | -48.5 | NC_001902.1 | + | 2819 | 0.75 | 0.386453 |
Target: 5'- gGGGGUACUcUCGUCaAUGUAUGUACAa -3' miRNA: 3'- -CCCUAUGGaAGCGGcUACAUGUAUGU- -5' |
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7439 | 3' | -48.5 | NC_001902.1 | + | 2729 | 1.12 | 0.00129 |
Target: 5'- aGGGAUACCUUCGCCGAUGUACAUACAu -3' miRNA: 3'- -CCCUAUGGAAGCGGCUACAUGUAUGU- -5' |
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7439 | 3' | -48.5 | NC_001902.1 | + | 2694 | 0.75 | 0.386453 |
Target: 5'- gGGGGUACUcUCGUCaAUGUAUGUACAa -3' miRNA: 3'- -CCCUAUGGaAGCGGcUACAUGUAUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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