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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7445 | 5' | -59.8 | NC_001902.1 | + | 25987 | 1.07 | 0.000182 |
|
Target: 5'- aCAGGGUCACCCUGACCCUAACCCCCUc -3' miRNA: 3'- -GUCCCAGUGGGACUGGGAUUGGGGGA- -5' |
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| 7445 | 5' | -59.8 | NC_001902.1 | + | 18303 | 0.71 | 0.121562 |
|
Target: 5'- --aGGUCACCCUGcaaugcgugGCCCUccccaagcccgggaaGACCCCCc -3' miRNA: 3'- gucCCAGUGGGAC---------UGGGA---------------UUGGGGGa -5' |
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| 7445 | 5' | -59.8 | NC_001902.1 | + | 8884 | 0.69 | 0.159845 |
|
Target: 5'- gCAGuaa-ACCCUGAUgCUGACCCCCa -3' miRNA: 3'- -GUCccagUGGGACUGgGAUUGGGGGa -5' |
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| 7445 | 5' | -59.8 | NC_001902.1 | + | 26033 | 0.72 | 0.095235 |
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Target: 5'- uUAGGGUCAgggUGACCCUGuCCCCCa -3' miRNA: 3'- -GUCCCAGUgggACUGGGAUuGGGGGa -5' |
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| 7445 | 5' | -59.8 | NC_001902.1 | + | 25668 | 0.69 | 0.1788 |
|
Target: 5'- gGGGGUUuuggauuuuuACCCUGACCCcugcgUggUCCCUg -3' miRNA: 3'- gUCCCAG----------UGGGACUGGG-----AuuGGGGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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