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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7447 | 5' | -47.7 | NC_001909.1 | + | 11113 | 0.66 | 0.929232 |
Target: 5'- cGCAA-UGGACACccaGACAGCcaugauugccuugAAGAACa -3' miRNA: 3'- -CGUUgACCUGUGuc-UUGUCG-------------UUCUUG- -5' |
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7447 | 5' | -47.7 | NC_001909.1 | + | 18462 | 0.66 | 0.909747 |
Target: 5'- gGCAACUGaacaacuuGACACcgaaAGGAC-GCAAGAAg -3' miRNA: 3'- -CGUUGAC--------CUGUG----UCUUGuCGUUCUUg -5' |
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7447 | 5' | -47.7 | NC_001909.1 | + | 1934 | 1.13 | 0.00141 |
Target: 5'- aGCAACUGGACACAGAACAGCAAGAACu -3' miRNA: 3'- -CGUUGACCUGUGUCUUGUCGUUCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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