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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 745 | 3' | -60.7 | AC_000020.1 | + | 9880 | 0.66 | 0.279037 |
|
Target: 5'- gCCCGCUuacaagGGGGUgcugcuccagaguUGcACCCCcGGGuGCAGa -3' miRNA: 3'- -GGGCGA------CCCCA-------------AU-UGGGGuCCC-CGUU- -5' |
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| 745 | 3' | -60.7 | AC_000020.1 | + | 15676 | 0.66 | 0.279754 |
|
Target: 5'- aCCaUGCUGGGGUgcagccgcACCUCAGGcaggaugcuguuGGCAGg -3' miRNA: 3'- -GG-GCGACCCCAau------UGGGGUCC------------CCGUU- -5' |
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| 745 | 3' | -60.7 | AC_000020.1 | + | 26699 | 0.66 | 0.261596 |
|
Target: 5'- cCuuGCUuguuauuaaaaaaagGGGGUUGuguggaagaCCCGGGGGCGu -3' miRNA: 3'- -GggCGA---------------CCCCAAUug-------GGGUCCCCGUu -5' |
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| 745 | 3' | -60.7 | AC_000020.1 | + | 21123 | 0.84 | 0.010903 |
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Target: 5'- gCCC-CUGGGGUUAACCCCAGcGGGUg- -3' miRNA: 3'- -GGGcGACCCCAAUUGGGGUC-CCCGuu -5' |
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| 745 | 3' | -60.7 | AC_000020.1 | + | 21068 | 1.08 | 0.000147 |
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Target: 5'- aCCCGCUGGGGUUAACCCCAGGGGCAAg -3' miRNA: 3'- -GGGCGACCCCAAUUGGGGUCCCCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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