miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
745 3' -60.7 AC_000020.1 + 9880 0.66 0.279037
Target:  5'- gCCCGCUuacaagGGGGUgcugcuccagaguUGcACCCCcGGGuGCAGa -3'
miRNA:   3'- -GGGCGA------CCCCA-------------AU-UGGGGuCCC-CGUU- -5'
745 3' -60.7 AC_000020.1 + 15676 0.66 0.279754
Target:  5'- aCCaUGCUGGGGUgcagccgcACCUCAGGcaggaugcuguuGGCAGg -3'
miRNA:   3'- -GG-GCGACCCCAau------UGGGGUCC------------CCGUU- -5'
745 3' -60.7 AC_000020.1 + 26699 0.66 0.261596
Target:  5'- cCuuGCUuguuauuaaaaaaagGGGGUUGuguggaagaCCCGGGGGCGu -3'
miRNA:   3'- -GggCGA---------------CCCCAAUug-------GGGUCCCCGUu -5'
745 3' -60.7 AC_000020.1 + 21123 0.84 0.010903
Target:  5'- gCCC-CUGGGGUUAACCCCAGcGGGUg- -3'
miRNA:   3'- -GGGcGACCCCAAUUGGGGUC-CCCGuu -5'
745 3' -60.7 AC_000020.1 + 21068 1.08 0.000147
Target:  5'- aCCCGCUGGGGUUAACCCCAGGGGCAAg -3'
miRNA:   3'- -GGGCGACCCCAAUUGGGGUCCCCGUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.