Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 745 | 5' | -43.1 | AC_000020.1 | + | 13192 | 0.66 | 0.998028 |
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Target: 5'- gCUUUGAUCcCGAGAcCAAGCuggucauGCcGGg -3' miRNA: 3'- -GAAACUAGuGUUUUaGUUCGu------CGuCC- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 9502 | 0.66 | 0.998028 |
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Target: 5'- uCUUUGA-CACcAGGUCcuaCAGCAGGc -3' miRNA: 3'- -GAAACUaGUGuUUUAGuucGUCGUCC- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 18351 | 0.66 | 0.99702 |
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Target: 5'- ---gGAUCuAUAAGGUU-GGCGGCGGGu -3' miRNA: 3'- gaaaCUAG-UGUUUUAGuUCGUCGUCC- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 663 | 0.67 | 0.994738 |
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Target: 5'- ---aGAUCguGCAGAGU-AGGCGGCgAGGg -3' miRNA: 3'- gaaaCUAG--UGUUUUAgUUCGUCG-UCC- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 15489 | 0.67 | 0.993719 |
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Target: 5'- ---cGGUCGCGGu-UCuGGCGGCAGcGg -3' miRNA: 3'- gaaaCUAGUGUUuuAGuUCGUCGUC-C- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 21153 | 0.67 | 0.993719 |
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Target: 5'- -cUUGAUUuuguGAUCaAAGCAGCAGa -3' miRNA: 3'- gaAACUAGuguuUUAG-UUCGUCGUCc -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 11701 | 0.69 | 0.975859 |
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Target: 5'- ---aGGUCACAGA----GGuCAGCAGGg -3' miRNA: 3'- gaaaCUAGUGUUUuaguUC-GUCGUCC- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 18893 | 0.73 | 0.892077 |
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Target: 5'- ---gGAUCAUAAGG-CGAGCccagGGCAGGa -3' miRNA: 3'- gaaaCUAGUGUUUUaGUUCG----UCGUCC- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 26259 | 0.77 | 0.686649 |
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Target: 5'- uCUUUGGgguUUGCAAAAaaGGGCAGCAGGa -3' miRNA: 3'- -GAAACU---AGUGUUUUagUUCGUCGUCC- -5' |
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| 745 | 5' | -43.1 | AC_000020.1 | + | 21103 | 1.12 | 0.00609 |
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Target: 5'- gCUUUGAUCACAAAAUCAAGCAGCAGGc -3' miRNA: 3'- -GAAACUAGUGUUUUAGUUCGUCGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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