Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
746 | 5' | -51.4 | AC_000020.1 | + | 17320 | 0.66 | 0.751869 |
Target: 5'- aGGGCAuuguaggcgguGCC-GCUguaaGGCUugaaGGAGGGGCCu -3' miRNA: 3'- -CCUGU-----------UGGaCGA----UCGA----UUUUCCCGGc -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 11068 | 0.66 | 0.740665 |
Target: 5'- --cCAGCCUGUUAGC-AAAA-GGCUGg -3' miRNA: 3'- ccuGUUGGACGAUCGaUUUUcCCGGC- -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 15622 | 0.66 | 0.740665 |
Target: 5'- cGGCugaucuuGCCUGUaGGCUu--GGGGCCc -3' miRNA: 3'- cCUGu------UGGACGaUCGAuuuUCCCGGc -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 2638 | 0.67 | 0.717889 |
Target: 5'- aGGAUuguACCUGCUcuauuauGCU-GGGGGGUCa -3' miRNA: 3'- -CCUGu--UGGACGAu------CGAuUUUCCCGGc -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 7967 | 0.67 | 0.694721 |
Target: 5'- uGGCAaagGCCUGUgcugauccugAGCUGGGaggcuAGGGCCa -3' miRNA: 3'- cCUGU---UGGACGa---------UCGAUUU-----UCCCGGc -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 24739 | 0.67 | 0.671272 |
Target: 5'- aGGACGAgcgagaccccCCUcCUAGCUGGAAuaugccgucGGGCCu -3' miRNA: 3'- -CCUGUU----------GGAcGAUCGAUUUU---------CCCGGc -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 11430 | 0.67 | 0.670094 |
Target: 5'- uGGGC-ACCUcGCUuaccagcAGCUu-AAGGGCUGa -3' miRNA: 3'- -CCUGuUGGA-CGA-------UCGAuuUUCCCGGC- -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 7772 | 0.68 | 0.635811 |
Target: 5'- -cACAACCUGCccGCccucaUAGAGGaGGCCGc -3' miRNA: 3'- ccUGUUGGACGauCG-----AUUUUC-CCGGC- -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 4805 | 0.68 | 0.623966 |
Target: 5'- cGugGGCCUGCacGCUGGuGAGGGUCc -3' miRNA: 3'- cCugUUGGACGauCGAUU-UUCCCGGc -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 12273 | 0.69 | 0.576785 |
Target: 5'- aGGACcGCCUagaGCUAGCU---GGGGUgGu -3' miRNA: 3'- -CCUGuUGGA---CGAUCGAuuuUCCCGgC- -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 17458 | 0.69 | 0.565095 |
Target: 5'- uGGCAGCCUaGCagAGCUGGGGGaugcgucuGGCCGu -3' miRNA: 3'- cCUGUUGGA-CGa-UCGAUUUUC--------CCGGC- -5' |
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746 | 5' | -51.4 | AC_000020.1 | + | 22355 | 1.11 | 0.000846 |
Target: 5'- uGGACAACCUGCUAGCUAAAAGGGCCGc -3' miRNA: 3'- -CCUGUUGGACGAUCGAUUUUCCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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