miRNA: 3'- -GGGUAc--UGGGCCUC-CUCUCGaCCGgU- -5'
miRNA: 3'- gGGUA-CUGG-GCCuCCU-CUCGaCCGGU- -5'
miRNA: 3'- -GGGUACUGGGCCUCCUCUCGACCGGU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7467 | 3' | -60.2 | NC_001918.1 | + | 1375 | 0.66 | 0.100152 |
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Target: 5'- cUCCGUaucGCCCGGGGaGGGAGUcGGCgGa -3' miRNA: 3'- -GGGUAc--UGGGCCUC-CUCUCGaCCGgU- -5' |
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| 7467 | 3' | -60.2 | NC_001918.1 | + | 2504 | 0.66 | 0.091043 |
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Target: 5'- gCCGUgGACCaUGGcGGAcGAGCcGGCCGa -3' miRNA: 3'- gGGUA-CUGG-GCCuCCU-CUCGaCCGGU- -5' |
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| 7467 | 3' | -60.2 | NC_001918.1 | + | 3589 | 1.1 | 1.5e-05 |
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Target: 5'- cCCCAUGACCCGGAGGAGAGCUGGCCAa -3' miRNA: 3'- -GGGUACUGGGCCUCCUCUCGACCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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