Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7472 | 3' | -63.2 | NC_001918.1 | + | 6938 | 0.66 | 0.063077 |
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Target: 5'- cGCCUggCCCAUGUCCaggCCCUCCAuGUUUg -3' miRNA: 3'- -CGGGa-GGGUGUAGGa--GGGGGGU-CGGA- -5' |
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| 7472 | 3' | -63.2 | NC_001918.1 | + | 1525 | 0.66 | 0.055511 |
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Target: 5'- gGCCUUCCCggcaGCAUCgaUUCCCUCgaUAGCCg -3' miRNA: 3'- -CGGGAGGG----UGUAG--GAGGGGG--GUCGGa -5' |
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| 7472 | 3' | -63.2 | NC_001918.1 | + | 178 | 0.67 | 0.048829 |
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Target: 5'- aGCUaggUCCACAUCCUCCCUUCcccugggagGGCCc -3' miRNA: 3'- -CGGga-GGGUGUAGGAGGGGGG---------UCGGa -5' |
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| 7472 | 3' | -63.2 | NC_001918.1 | + | 4060 | 0.7 | 0.028192 |
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Target: 5'- uCCCUCCCcaACAccggCUUCUCCCUGGCCc -3' miRNA: 3'- cGGGAGGG--UGUa---GGAGGGGGGUCGGa -5' |
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| 7472 | 3' | -63.2 | NC_001918.1 | + | 3482 | 0.71 | 0.020362 |
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Target: 5'- aUCC-CCUACA-CCUCCCCCCucucuGCCa -3' miRNA: 3'- cGGGaGGGUGUaGGAGGGGGGu----CGGa -5' |
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| 7472 | 3' | -63.2 | NC_001918.1 | + | 216 | 1.09 | 0.000008 |
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Target: 5'- cGCCCUCCCACAUCCUCCCCCCAGCCUg -3' miRNA: 3'- -CGGGAGGGUGUAGGAGGGGGGUCGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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