Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7474 | 3' | -61.3 | NC_001918.1 | + | 6397 | 0.66 | 0.098751 |
Target: 5'- cCCCGGAgucgUCGUCUguaagcaGGCGuacggauACCGCGCCg- -3' miRNA: 3'- -GGGCCU----GGUAGG-------CCGC-------UGGCGUGGac -5' |
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7474 | 3' | -61.3 | NC_001918.1 | + | 3840 | 0.66 | 0.093365 |
Target: 5'- aCUGGGCCAUCCgcaaGGUGGCCcccgacggcaGUGCCa- -3' miRNA: 3'- gGGCCUGGUAGG----CCGCUGG----------CGUGGac -5' |
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7474 | 3' | -61.3 | NC_001918.1 | + | 2402 | 0.67 | 0.074959 |
Target: 5'- uCCUGG-CCAgccaCGGCaGAuccgaaagugcCCGCACCUGg -3' miRNA: 3'- -GGGCCuGGUag--GCCG-CU-----------GGCGUGGAC- -5' |
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7474 | 3' | -61.3 | NC_001918.1 | + | 6821 | 0.7 | 0.040934 |
Target: 5'- aCCGGGCCAgggcCCGGUGA-CGuCGCCUu -3' miRNA: 3'- gGGCCUGGUa---GGCCGCUgGC-GUGGAc -5' |
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7474 | 3' | -61.3 | NC_001918.1 | + | 1722 | 1.1 | 1.3e-05 |
Target: 5'- aCCCGGACCAUCCGGCGACCGCACCUGg -3' miRNA: 3'- -GGGCCUGGUAGGCCGCUGGCGUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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