Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7482 | 3' | -55.3 | NC_001918.1 | + | 8194 | 0.67 | 0.169258 |
Target: 5'- aCUgCACCCGcGGAccaGGGCCGUCUuUGa -3' miRNA: 3'- gGA-GUGGGU-CCUug-UCCGGUAGGuAC- -5' |
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7482 | 3' | -55.3 | NC_001918.1 | + | 4915 | 0.7 | 0.104477 |
Target: 5'- uCCaUCGCCCAGGA---GGCCAaaUCCGg- -3' miRNA: 3'- -GG-AGUGGGUCCUuguCCGGU--AGGUac -5' |
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7482 | 3' | -55.3 | NC_001918.1 | + | 6937 | 0.73 | 0.061461 |
Target: 5'- gCCUgGCCCAuGucCAGGCCcUCCAUGu -3' miRNA: 3'- -GGAgUGGGUcCuuGUCCGGuAGGUAC- -5' |
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7482 | 3' | -55.3 | NC_001918.1 | + | 6867 | 1.11 | 4.3e-05 |
Target: 5'- uCCUCACCCAGGAACAGGCCAUCCAUGg -3' miRNA: 3'- -GGAGUGGGUCCUUGUCCGGUAGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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