Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 749 | 3' | -55.7 | AC_000020.1 | + | 7420 | 0.66 | 0.548693 |
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Target: 5'- uUGGGCgggGCCC-CAAUGGGGGUGAa -3' miRNA: 3'- gGCCUGag-UGGGaGUUGCUCCCGUUc -5' |
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| 749 | 3' | -55.7 | AC_000020.1 | + | 7708 | 0.66 | 0.537664 |
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Target: 5'- gCCGcGGC-C-UCCUCuAUGAGGGCGGGc -3' miRNA: 3'- -GGC-CUGaGuGGGAGuUGCUCCCGUUC- -5' |
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| 749 | 3' | -55.7 | AC_000020.1 | + | 23096 | 0.7 | 0.332275 |
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Target: 5'- gUGGGCagCGCgCaggUCAugGAGGGCAGGa -3' miRNA: 3'- gGCCUGa-GUGgG---AGUugCUCCCGUUC- -5' |
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| 749 | 3' | -55.7 | AC_000020.1 | + | 25518 | 0.7 | 0.311183 |
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Target: 5'- -aGGAUggccuguacaUCGCCCUCAuuaaggagagcaacuACGAGGGCGc- -3' miRNA: 3'- ggCCUG----------AGUGGGAGU---------------UGCUCCCGUuc -5' |
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| 749 | 3' | -55.7 | AC_000020.1 | + | 26829 | 0.71 | 0.277841 |
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Target: 5'- gCUGGcuuguaACUUGCCCUCGuUGAGGGUGAGu -3' miRNA: 3'- -GGCC------UGAGUGGGAGUuGCUCCCGUUC- -5' |
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| 749 | 3' | -55.7 | AC_000020.1 | + | 14256 | 0.71 | 0.270671 |
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Target: 5'- gCGGGCUCGgCCUUGACGGcGGCGGc -3' miRNA: 3'- gGCCUGAGUgGGAGUUGCUcCCGUUc -5' |
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| 749 | 3' | -55.7 | AC_000020.1 | + | 26761 | 1.1 | 0.000406 |
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Target: 5'- cCCGGACUCACCCUCAACGAGGGCAAGu -3' miRNA: 3'- -GGCCUGAGUGGGAGUUGCUCCCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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