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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
749 | 5' | -53.5 | AC_000020.1 | + | 7707 | 0.66 | 0.65177 |
Target: 5'- uGCCgCGGCCUCcUCuaugagggcggGCAGGUugUGGGc -3' miRNA: 3'- cCGG-GCCGGAGuAG-----------UGUUUAugGCUC- -5' |
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749 | 5' | -53.5 | AC_000020.1 | + | 16231 | 0.68 | 0.525885 |
Target: 5'- aGGCCCcGCCguUCAUgcCGCuGGUGCCGAu -3' miRNA: 3'- -CCGGGcCGG--AGUA--GUGuUUAUGGCUc -5' |
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749 | 5' | -53.5 | AC_000020.1 | + | 26803 | 1.12 | 0.000474 |
Target: 5'- gGGCCCGGCCUCAUCACAAAUACCGAGg -3' miRNA: 3'- -CCGGGCCGGAGUAGUGUUUAUGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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