miRNA: 3'- cuaaUCCUCUCAGuC-CCGG-UCCUGGG- -5'
miRNA: 3'- cUAAUCCucUCAGUCCCGGU--CCUGGG- -5'
miRNA: 3'- cuaaUCCUCUCAGucCCGGucCUGGG- -5'
miRNA: 3'- -CUAAUCCUCUCAGUCCCGGUCCUGGG- -5'
miRNA: 3'- cuaaUCCUCU---------------CAGUCCcGG-UCCUGGG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7520 | 5' | -55.6 | NC_001940.1 | + | 1196 | 0.66 | 0.196078 |
|
Target: 5'- ---aAGGGGGGUCuGuGGCUaAGGAUCUc -3' miRNA: 3'- cuaaUCCUCUCAGuC-CCGG-UCCUGGG- -5' |
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| 7520 | 5' | -55.6 | NC_001940.1 | + | 1626 | 0.67 | 0.147806 |
|
Target: 5'- aAUUAGGuuAGUUAGGGCCAcuGGGUCUu -3' miRNA: 3'- cUAAUCCucUCAGUCCCGGU--CCUGGG- -5' |
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| 7520 | 5' | -55.6 | NC_001940.1 | + | 2458 | 0.68 | 0.143166 |
|
Target: 5'- ---gGGGGGGGUCcuGGCCcuGACUCu -3' miRNA: 3'- cuaaUCCUCUCAGucCCGGucCUGGG- -5' |
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| 7520 | 5' | -55.6 | NC_001940.1 | + | 2419 | 1.11 | 0.000039 |
|
Target: 5'- aGAUUAGGAGAGUCAGGGCCAGGACCCc -3' miRNA: 3'- -CUAAUCCUCUCAGUCCCGGUCCUGGG- -5' |
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| 7520 | 5' | -55.6 | NC_001940.1 | + | 1387 | 0.67 | 0.154532 |
|
Target: 5'- ---cGGGAGGucacgggccaccgccGUCAGGuCCuAGGACCCc -3' miRNA: 3'- cuaaUCCUCU---------------CAGUCCcGG-UCCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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