Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
753 | 3' | -55.7 | NC_000852.3 | + | 227251 | 0.66 | 0.983036 |
Target: 5'- cUCCAGuugUGGCAGCuucGCGGGUgcugggagaggcccgCUUGGCu -3' miRNA: 3'- -AGGUUcu-GCUGUCG---CGCCCA---------------GAACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 241273 | 0.66 | 0.982293 |
Target: 5'- uUUCGGGGCGGguuuggguuuCGGgGCGGGUUUggguuucggGGCa -3' miRNA: 3'- -AGGUUCUGCU----------GUCgCGCCCAGAa--------CCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 150691 | 0.66 | 0.98033 |
Target: 5'- aCguAGACGAUcuAGCGCGaGGUCUacucaucgucaUGGa -3' miRNA: 3'- aGguUCUGCUG--UCGCGC-CCAGA-----------ACCg -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 197938 | 0.66 | 0.978208 |
Target: 5'- ---cGGGCuuUGGUGCGGGUUUUGGUg -3' miRNA: 3'- agguUCUGcuGUCGCGCCCAGAACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 197693 | 0.66 | 0.978208 |
Target: 5'- uUCgGAG-CGGguuuCGGUGCGGGUUUaggGGCg -3' miRNA: 3'- -AGgUUCuGCU----GUCGCGCCCAGAa--CCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 188496 | 0.66 | 0.978208 |
Target: 5'- gUCCugGcGCGGguuuaGGCGCGGGUUUaGGUg -3' miRNA: 3'- -AGGuuC-UGCUg----UCGCGCCCAGAaCCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 217323 | 0.66 | 0.978208 |
Target: 5'- uUCCGucuaACG-CAGCGCGGGcUCcagaUUGGUa -3' miRNA: 3'- -AGGUuc--UGCuGUCGCGCCC-AG----AACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 122090 | 0.67 | 0.965014 |
Target: 5'- cUCCAAGAuaCGACA-CGUaGGagaUCUUGGCa -3' miRNA: 3'- -AGGUUCU--GCUGUcGCGcCC---AGAACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 239969 | 0.68 | 0.958425 |
Target: 5'- aUCCGAGAgcaaGAcCGGUGUGGGUCUacGGa -3' miRNA: 3'- -AGGUUCUg---CU-GUCGCGCCCAGAa-CCg -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 277560 | 0.68 | 0.958075 |
Target: 5'- -aCAGGACGACgucgugcauaugaAGacaGCGGGUgagaUUGGCg -3' miRNA: 3'- agGUUCUGCUG-------------UCg--CGCCCAg---AACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 197979 | 0.68 | 0.954831 |
Target: 5'- cUCAGGAC--CAGUcgauGCGGGUUUUGGUg -3' miRNA: 3'- aGGUUCUGcuGUCG----CGCCCAGAACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 241109 | 0.68 | 0.951032 |
Target: 5'- -gCAGGuCGuauaGGCGCGGGUUcUGGUg -3' miRNA: 3'- agGUUCuGCug--UCGCGCCCAGaACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 241165 | 0.68 | 0.947025 |
Target: 5'- uUCUGGcGCGGguucUGGCGCGGGUUcUGGCg -3' miRNA: 3'- -AGGUUcUGCU----GUCGCGCCCAGaACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 302675 | 0.68 | 0.942375 |
Target: 5'- uUCC-AGAC-ACGGaaccuaaUGCGGGUCUUGGa -3' miRNA: 3'- -AGGuUCUGcUGUC-------GCGCCCAGAACCg -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 210030 | 0.69 | 0.938379 |
Target: 5'- gCCGAGuACuGCGGCGgGGGaagUGGCa -3' miRNA: 3'- aGGUUC-UGcUGUCGCgCCCagaACCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 94677 | 0.69 | 0.923802 |
Target: 5'- gCCAGGGCGAguGUGa-GGUCacGGCg -3' miRNA: 3'- aGGUUCUGCUguCGCgcCCAGaaCCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 158776 | 0.69 | 0.918512 |
Target: 5'- gCCuuGGuACGGguuuCGGCGCGGGUUUcGGCg -3' miRNA: 3'- aGGu-UC-UGCU----GUCGCGCCCAGAaCCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 123023 | 0.7 | 0.907289 |
Target: 5'- uUUCGGcGCGGguuuCGGCGCGGGUUUcGGCg -3' miRNA: 3'- -AGGUUcUGCU----GUCGCGCCCAGAaCCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 197574 | 0.7 | 0.901359 |
Target: 5'- uUUCGGcgcuGGCGuCGGCGCGGGUUUcGGUa -3' miRNA: 3'- -AGGUU----CUGCuGUCGCGCCCAGAaCCG- -5' |
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753 | 3' | -55.7 | NC_000852.3 | + | 251494 | 0.7 | 0.882319 |
Target: 5'- gCUggGGgaGGCAGCGCgacGGGUCUUagaGGCg -3' miRNA: 3'- aGGuuCUg-CUGUCGCG---CCCAGAA---CCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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