Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 753 | 5' | -50.4 | NC_000852.3 | + | 278611 | 0.66 | 0.999664 |
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Target: 5'- -gGCCU-GAAAGAUagGCCgauggCAUCUUGa -3' miRNA: 3'- agCGGAgCUUUCUG--CGGaa---GUAGAAC- -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 60049 | 0.67 | 0.999583 |
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Target: 5'- --uUCUCGAAAGACGUCcUCAUUUg- -3' miRNA: 3'- agcGGAGCUUUCUGCGGaAGUAGAac -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 155899 | 0.67 | 0.999485 |
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Target: 5'- gUCGCUcacuUCGAGaauuuccuuGGGCGCCUcaUCGUCa-- -3' miRNA: 3'- -AGCGG----AGCUU---------UCUGCGGA--AGUAGaac -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 17701 | 0.67 | 0.999227 |
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Target: 5'- gCGCUcgCGGAAGACGCCaaUAUCa-- -3' miRNA: 3'- aGCGGa-GCUUUCUGCGGaaGUAGaac -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 170063 | 0.68 | 0.998866 |
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Target: 5'- gCGaaCCUCGugauGGACGUCUUCAUCc-- -3' miRNA: 3'- aGC--GGAGCuu--UCUGCGGAAGUAGaac -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 266908 | 0.7 | 0.991275 |
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Target: 5'- aCG-CUCGuAAGACGCCUUCAaaaaUCUg- -3' miRNA: 3'- aGCgGAGCuUUCUGCGGAAGU----AGAac -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 250019 | 0.74 | 0.930044 |
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Target: 5'- aCGCgCUCGAaaagaAAGACGUCUUCAUUUg- -3' miRNA: 3'- aGCG-GAGCU-----UUCUGCGGAAGUAGAac -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 8479 | 0.76 | 0.867321 |
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Target: 5'- gUCGCCgUGGgaaagcAAGACGCCUUCAUCa-- -3' miRNA: 3'- -AGCGGaGCU------UUCUGCGGAAGUAGaac -5' |
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| 753 | 5' | -50.4 | NC_000852.3 | + | 81702 | 1.11 | 0.016653 |
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Target: 5'- aUCGCCUCGAAAGACGCCUUCAUCUUGg -3' miRNA: 3'- -AGCGGAGCUUUCUGCGGAAGUAGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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