miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7531 3' -54.5 NC_001944.1 + 501 1.14 0.000002
Target:  5'- uCCGGACAUCACGGCAGCAACAGCUCCa -3'
miRNA:   3'- -GGCCUGUAGUGCCGUCGUUGUCGAGG- -5'
7531 3' -54.5 NC_001944.1 + 68 0.68 0.035792
Target:  5'- gCGGGCAccucCGCaaGGCAGCcAAUAGCUgCg -3'
miRNA:   3'- gGCCUGUa---GUG--CCGUCG-UUGUCGAgG- -5'
7531 3' -54.5 NC_001944.1 + 362 0.66 0.048593
Target:  5'- --uGACcUCGCGGCAGCAuuuucuuaaGCGcGCUCa -3'
miRNA:   3'- ggcCUGuAGUGCCGUCGU---------UGU-CGAGg -5'
7531 3' -54.5 NC_001944.1 + 1903 0.66 0.050478
Target:  5'- aUGuGACGUCuGCGGUAGUAugGGCgggcauaCCg -3'
miRNA:   3'- gGC-CUGUAG-UGCCGUCGUugUCGa------GG- -5'
7531 3' -54.5 NC_001944.1 + 1165 0.66 0.056571
Target:  5'- cCCGacacGACGaaCACGGUAGCGACgaaGGCgCCa -3'
miRNA:   3'- -GGC----CUGUa-GUGCCGUCGUUG---UCGaGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.