miRNA: 3'- gaAGUCGGAACU------------ACAGUUUUUGAgcu -5'
miRNA: 3'- -GAAGUCgGAACUACAGUUUUUGAGCU- -5'
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7534 | 3' | -44.5 | NC_001946.1 | + | 4294 | 0.71 | 0.471043 |
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Target: 5'- -aUCAGCUUUGAaauccuugaaguUGUCAAAGACUg-- -3' miRNA: 3'- gaAGUCGGAACU------------ACAGUUUUUGAgcu -5' |
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| 7534 | 3' | -44.5 | NC_001946.1 | + | 3390 | 0.96 | 0.010235 |
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Target: 5'- aCUUCAG-CUUGAUGUCAAAAACUCGAa -3' miRNA: 3'- -GAAGUCgGAACUACAGUUUUUGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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