Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 754 | 3' | -50.4 | NC_000852.3 | + | 317101 | 0.66 | 0.999745 |
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Target: 5'- aUCCCGGGcGUAUGGCGuauaugaUGGUCuAGUg- -3' miRNA: 3'- aGGGGCUC-UAUACCGU-------ACUAG-UCAaa -5' |
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| 754 | 3' | -50.4 | NC_000852.3 | + | 250670 | 0.67 | 0.999086 |
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Target: 5'- cUCCCGAGAUAUGGU--GAaguuccccaaaguUCAGUg- -3' miRNA: 3'- aGGGGCUCUAUACCGuaCU-------------AGUCAaa -5' |
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| 754 | 3' | -50.4 | NC_000852.3 | + | 117451 | 0.68 | 0.997763 |
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Target: 5'- uUCCCCGaAGAUAUGGacucgGAUUGGa-- -3' miRNA: 3'- -AGGGGC-UCUAUACCgua--CUAGUCaaa -5' |
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| 754 | 3' | -50.4 | NC_000852.3 | + | 308684 | 0.68 | 0.996348 |
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Target: 5'- aUCaCuuGGGAUAUGGauuuuauggAUGAUCAGUUUg -3' miRNA: 3'- -AG-GggCUCUAUACCg--------UACUAGUCAAA- -5' |
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| 754 | 3' | -50.4 | NC_000852.3 | + | 109568 | 0.73 | 0.955961 |
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Target: 5'- aCCCCGGGGU-UGGCggGuUCAGUUUu -3' miRNA: 3'- aGGGGCUCUAuACCGuaCuAGUCAAA- -5' |
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| 754 | 3' | -50.4 | NC_000852.3 | + | 245119 | 0.75 | 0.905767 |
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Target: 5'- aUCCaCCGcaacuGGUGUGGCAUGGUCGGc-- -3' miRNA: 3'- -AGG-GGCu----CUAUACCGUACUAGUCaaa -5' |
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| 754 | 3' | -50.4 | NC_000852.3 | + | 117843 | 1.06 | 0.028269 |
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Target: 5'- uUCCCCGAGAUAUGGCAUGAUCAGUUUc -3' miRNA: 3'- -AGGGGCUCUAUACCGUACUAGUCAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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