Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 754 | 5' | -50.7 | NC_000852.3 | + | 70650 | 0.66 | 0.999865 |
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Target: 5'- uUCGGuUuuGGGUCUAUGUCUauuUCGGGa- -3' miRNA: 3'- -GGUU-AggCUCAGGUAUAGA---AGCCCcu -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 131533 | 0.66 | 0.999769 |
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Target: 5'- -aAAUCCGGGUcuagacCCAUAUCaUuuuuaaggucuucgUCGGGGGg -3' miRNA: 3'- ggUUAGGCUCA------GGUAUAG-A--------------AGCCCCU- -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 117489 | 0.66 | 0.999729 |
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Target: 5'- aCUGAUCaug--CCAUAUC-UCGGGGAa -3' miRNA: 3'- -GGUUAGgcucaGGUAUAGaAGCCCCU- -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 189665 | 0.67 | 0.999233 |
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Target: 5'- cCCGAUCCuGGUCCAcccgUAUUaaaaccCGGGGGu -3' miRNA: 3'- -GGUUAGGcUCAGGU----AUAGaa----GCCCCU- -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 22684 | 0.68 | 0.99888 |
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Target: 5'- ---uUCUGAG-CCAUGUCUUgacauaccugCGGGGGu -3' miRNA: 3'- gguuAGGCUCaGGUAUAGAA----------GCCCCU- -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 106504 | 0.68 | 0.998096 |
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Target: 5'- aCAAUC---GUCacuGUGUCUUCGGGGAa -3' miRNA: 3'- gGUUAGgcuCAGg--UAUAGAAGCCCCU- -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 326595 | 0.68 | 0.998096 |
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Target: 5'- gUAAUCau-GUCUAUGUCUUCuGGGAa -3' miRNA: 3'- gGUUAGgcuCAGGUAUAGAAGcCCCU- -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 221376 | 0.7 | 0.995125 |
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Target: 5'- gCCGAguUCC-AGUCCAUggUUUCGGGuGAa -3' miRNA: 3'- -GGUU--AGGcUCAGGUAuaGAAGCCC-CU- -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 108943 | 0.74 | 0.950851 |
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Target: 5'- -aAAUCCGAuUCCAUGccCUUCGGGGu -3' miRNA: 3'- ggUUAGGCUcAGGUAUa-GAAGCCCCu -5' |
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| 754 | 5' | -50.7 | NC_000852.3 | + | 117808 | 1.12 | 0.015972 |
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Target: 5'- uCCAAUCCGAGUCCAUAUCUUCGGGGAa -3' miRNA: 3'- -GGUUAGGCUCAGGUAUAGAAGCCCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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