Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 755 | 3' | -58.1 | NC_000852.3 | + | 142541 | 0.68 | 0.898357 |
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Target: 5'- nCCCCAGUGUCGCGcaaAGAaCCuacaccaccaaCCGAg -3' miRNA: 3'- uGGGGUCACAGCGCa--UCUaGG-----------GGCUg -5' |
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| 755 | 3' | -58.1 | NC_000852.3 | + | 144360 | 0.74 | 0.576232 |
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Target: 5'- aACCCCAGUGUCGCGcaaAGAaccuacacccaagacUCCCacauCGGCa -3' miRNA: 3'- -UGGGGUCACAGCGCa--UCU---------------AGGG----GCUG- -5' |
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| 755 | 3' | -58.1 | NC_000852.3 | + | 264700 | 0.76 | 0.454252 |
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Target: 5'- uACCCCAGaUGUCGCGgucGUCCUCGAa -3' miRNA: 3'- -UGGGGUC-ACAGCGCaucUAGGGGCUg -5' |
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| 755 | 3' | -58.1 | NC_000852.3 | + | 144384 | 0.83 | 0.192359 |
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Target: 5'- aACCCCAucGUCGCGUAGAUCCCCa-- -3' miRNA: 3'- -UGGGGUcaCAGCGCAUCUAGGGGcug -5' |
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| 755 | 3' | -58.1 | NC_000852.3 | + | 144432 | 0.84 | 0.171012 |
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Target: 5'- aACCCCAGUGUCGCGUAaAUCCCCn-- -3' miRNA: 3'- -UGGGGUCACAGCGCAUcUAGGGGcug -5' |
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| 755 | 3' | -58.1 | NC_000852.3 | + | 144408 | 0.89 | 0.086471 |
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Target: 5'- aACCUCAGUGUUGCGUAGAUCCCCa-- -3' miRNA: 3'- -UGGGGUCACAGCGCAUCUAGGGGcug -5' |
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| 755 | 3' | -58.1 | NC_000852.3 | + | 142566 | 1.08 | 0.005274 |
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Target: 5'- aACCCCAGUGUCGCGUAGAUCCCCGACc -3' miRNA: 3'- -UGGGGUCACAGCGCAUCUAGGGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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