miRNA display CGI


Results 1 - 8 of 8 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
756 3' -50 NC_000852.3 + 221005 0.66 0.999677
Target:  5'- -----gGCgUCUGGCGAuguuauuCUGGGGGa -3'
miRNA:   3'- aauuaaCG-AGACCGCUuau----GGUCCCC- -5'
756 3' -50 NC_000852.3 + 286202 0.67 0.999596
Target:  5'- ---uUUG-UCgaaGCGggUGCCGGGGGc -3'
miRNA:   3'- aauuAACgAGac-CGCuuAUGGUCCCC- -5'
756 3' -50 NC_000852.3 + 270285 0.67 0.999383
Target:  5'- ------cUUUUGGUucGggUGCCAGGGGa -3'
miRNA:   3'- aauuaacGAGACCG--CuuAUGGUCCCC- -5'
756 3' -50 NC_000852.3 + 145878 0.68 0.998652
Target:  5'- -----cGCUCUcGGau--UACCAGGGGa -3'
miRNA:   3'- aauuaaCGAGA-CCgcuuAUGGUCCCC- -5'
756 3' -50 NC_000852.3 + 37802 0.68 0.998069
Target:  5'- -gGAggGCUCgGGgGAggGCuCGGGGGa -3'
miRNA:   3'- aaUUaaCGAGaCCgCUuaUG-GUCCCC- -5'
756 3' -50 NC_000852.3 + 37838 0.68 0.998069
Target:  5'- -gGAggGCUCgGGgGAggGCuCGGGGGa -3'
miRNA:   3'- aaUUaaCGAGaCCgCUuaUG-GUCCCC- -5'
756 3' -50 NC_000852.3 + 100473 0.7 0.99114
Target:  5'- ---cUUGUUgUGGCGuuuUACCGGGGa -3'
miRNA:   3'- aauuAACGAgACCGCuu-AUGGUCCCc -5'
756 3' -50 NC_000852.3 + 157680 1.1 0.017533
Target:  5'- aUUAAUUGCUCUGGCGAAUACCAGGGGg -3'
miRNA:   3'- -AAUUAACGAGACCGCUUAUGGUCCCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.