Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
756 | 3' | -50 | NC_000852.3 | + | 221005 | 0.66 | 0.999677 |
Target: 5'- -----gGCgUCUGGCGAuguuauuCUGGGGGa -3' miRNA: 3'- aauuaaCG-AGACCGCUuau----GGUCCCC- -5' |
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756 | 3' | -50 | NC_000852.3 | + | 286202 | 0.67 | 0.999596 |
Target: 5'- ---uUUG-UCgaaGCGggUGCCGGGGGc -3' miRNA: 3'- aauuAACgAGac-CGCuuAUGGUCCCC- -5' |
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756 | 3' | -50 | NC_000852.3 | + | 270285 | 0.67 | 0.999383 |
Target: 5'- ------cUUUUGGUucGggUGCCAGGGGa -3' miRNA: 3'- aauuaacGAGACCG--CuuAUGGUCCCC- -5' |
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756 | 3' | -50 | NC_000852.3 | + | 145878 | 0.68 | 0.998652 |
Target: 5'- -----cGCUCUcGGau--UACCAGGGGa -3' miRNA: 3'- aauuaaCGAGA-CCgcuuAUGGUCCCC- -5' |
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756 | 3' | -50 | NC_000852.3 | + | 37802 | 0.68 | 0.998069 |
Target: 5'- -gGAggGCUCgGGgGAggGCuCGGGGGa -3' miRNA: 3'- aaUUaaCGAGaCCgCUuaUG-GUCCCC- -5' |
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756 | 3' | -50 | NC_000852.3 | + | 37838 | 0.68 | 0.998069 |
Target: 5'- -gGAggGCUCgGGgGAggGCuCGGGGGa -3' miRNA: 3'- aaUUaaCGAGaCCgCUuaUG-GUCCCC- -5' |
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756 | 3' | -50 | NC_000852.3 | + | 100473 | 0.7 | 0.99114 |
Target: 5'- ---cUUGUUgUGGCGuuuUACCGGGGa -3' miRNA: 3'- aauuAACGAgACCGCuu-AUGGUCCCc -5' |
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756 | 3' | -50 | NC_000852.3 | + | 157680 | 1.1 | 0.017533 |
Target: 5'- aUUAAUUGCUCUGGCGAAUACCAGGGGg -3' miRNA: 3'- -AAUUAACGAGACCGCUUAUGGUCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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