Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 756 | 5' | -58.4 | NC_000852.3 | + | 60317 | 0.71 | 0.745972 |
|
Target: 5'- gUCgUCGagGUCGCCAUGAUGUGUAUUa -3' miRNA: 3'- -GGgGGCagCGGCGGUGCUGCACAUAG- -5' |
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| 756 | 5' | -58.4 | NC_000852.3 | + | 157646 | 1.11 | 0.003665 |
|
Target: 5'- aCCCCCGUCGCCGCCACGACGUGUAUCu -3' miRNA: 3'- -GGGGGCAGCGGCGGUGCUGCACAUAG- -5' |
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| 756 | 5' | -58.4 | NC_000852.3 | + | 251796 | 0.69 | 0.866403 |
|
Target: 5'- gCUCCGUgCGCCGUCAUGcucguCGUGUGa- -3' miRNA: 3'- gGGGGCA-GCGGCGGUGCu----GCACAUag -5' |
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| 756 | 5' | -58.4 | NC_000852.3 | + | 254329 | 0.66 | 0.958964 |
|
Target: 5'- -aCCCGUCGUacuCGCCACGgaaGUGUu-- -3' miRNA: 3'- ggGGGCAGCG---GCGGUGCug-CACAuag -5' |
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| 756 | 5' | -58.4 | NC_000852.3 | + | 286037 | 0.67 | 0.939742 |
|
Target: 5'- -aCCCGUCGgCGCCGgcuaaGACGgggGUCa -3' miRNA: 3'- ggGGGCAGCgGCGGUg----CUGCacaUAG- -5' |
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| 756 | 5' | -58.4 | NC_000852.3 | + | 286394 | 0.7 | 0.829796 |
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Target: 5'- aCCCCCGUCuuaGCCGgCGcCGACGgguUGUGg- -3' miRNA: 3'- -GGGGGCAG---CGGCgGU-GCUGC---ACAUag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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