Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7562 | 5' | -60.6 | NC_001962.1 | + | 1592 | 0.66 | 0.663382 |
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Target: 5'- uGCCGgCUGaaauGUUACCaCCGCCugCAc -3' miRNA: 3'- gCGGCaGACg---UAGUGGcGGCGGugGU- -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 1907 | 0.66 | 0.653362 |
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Target: 5'- cCGCCaucaucgauGUCUGaAUUGCCGCCcgcuCCACCAa -3' miRNA: 3'- -GCGG---------CAGACgUAGUGGCGGc---GGUGGU- -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 93245 | 0.66 | 0.643326 |
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Target: 5'- gGCCGUCUGCG-CACgGCgC-CCGCgGa -3' miRNA: 3'- gCGGCAGACGUaGUGgCG-GcGGUGgU- -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 30424 | 0.67 | 0.623237 |
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Target: 5'- uGUCGUCgGCAagcaCGCCGCCGUUgggucgGCCAu -3' miRNA: 3'- gCGGCAGaCGUa---GUGGCGGCGG------UGGU- -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 84363 | 0.67 | 0.573249 |
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Target: 5'- uCGCCGUacgGCAUCGCUuucagGCUG-CACCAa -3' miRNA: 3'- -GCGGCAga-CGUAGUGG-----CGGCgGUGGU- -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 112257 | 0.7 | 0.431838 |
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Target: 5'- aCGCCuGUC-GCcaacUCGCCGCUGUCGCCu -3' miRNA: 3'- -GCGG-CAGaCGu---AGUGGCGGCGGUGGu -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 10608 | 0.72 | 0.327267 |
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Target: 5'- aGUCGUgUUGCGUCGUCGUCGCCGCUAu -3' miRNA: 3'- gCGGCA-GACGUAGUGGCGGCGGUGGU- -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 121920 | 0.75 | 0.231393 |
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Target: 5'- aCGCCGUCacucccagcaGCAgcaGCCGCCGCCACa- -3' miRNA: 3'- -GCGGCAGa---------CGUag-UGGCGGCGGUGgu -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 103432 | 0.75 | 0.220566 |
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Target: 5'- cCGCCGcCaccGCcgcCGCCGCCGCCGCCGc -3' miRNA: 3'- -GCGGCaGa--CGua-GUGGCGGCGGUGGU- -5' |
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| 7562 | 5' | -60.6 | NC_001962.1 | + | 1697 | 1.08 | 0.001087 |
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Target: 5'- cCGCCGUCUGCAUCACCGCCGCCACCAc -3' miRNA: 3'- -GCGGCAGACGUAGUGGCGGCGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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