miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
7563 3' -54.9 NC_001962.1 + 60661 0.66 0.919438
Target:  5'- uCAGCUUGgccgUCCAGUacaugGUGGCGGAAuCGg -3'
miRNA:   3'- -GUUGAGCa---GGGUCGa----CGCCGCUUU-GC- -5'
7563 3' -54.9 NC_001962.1 + 30268 0.66 0.913572
Target:  5'- gCAAUUUGUaauuaUCCA-UUGUGGCGAGACGg -3'
miRNA:   3'- -GUUGAGCA-----GGGUcGACGCCGCUUUGC- -5'
7563 3' -54.9 NC_001962.1 + 78355 0.66 0.901106
Target:  5'- ---aUCGUUUCAgGCUGCGGCccAGCGa -3'
miRNA:   3'- guugAGCAGGGU-CGACGCCGcuUUGC- -5'
7563 3' -54.9 NC_001962.1 + 49624 0.67 0.887677
Target:  5'- aCGAUUUG--CCGGCUGCGGCGccGACa -3'
miRNA:   3'- -GUUGAGCagGGUCGACGCCGCu-UUGc -5'
7563 3' -54.9 NC_001962.1 + 23951 0.68 0.833614
Target:  5'- aAACUUG-CCCAGCuUGCGGCccuuuGAAAUc -3'
miRNA:   3'- gUUGAGCaGGGUCG-ACGCCG-----CUUUGc -5'
7563 3' -54.9 NC_001962.1 + 81532 0.69 0.770333
Target:  5'- aCAACgcugccauagCGUCUCAGUaGCGGCGGgaAACGc -3'
miRNA:   3'- -GUUGa---------GCAGGGUCGaCGCCGCU--UUGC- -5'
7563 3' -54.9 NC_001962.1 + 13092 1.09 0.003251
Target:  5'- aCAACUCGUCCCAGCUGCGGCGAAACGu -3'
miRNA:   3'- -GUUGAGCAGGGUCGACGCCGCUUUGC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.