Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7563 | 3' | -54.9 | NC_001962.1 | + | 60661 | 0.66 | 0.919438 |
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Target: 5'- uCAGCUUGgccgUCCAGUacaugGUGGCGGAAuCGg -3' miRNA: 3'- -GUUGAGCa---GGGUCGa----CGCCGCUUU-GC- -5' |
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| 7563 | 3' | -54.9 | NC_001962.1 | + | 30268 | 0.66 | 0.913572 |
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Target: 5'- gCAAUUUGUaauuaUCCA-UUGUGGCGAGACGg -3' miRNA: 3'- -GUUGAGCA-----GGGUcGACGCCGCUUUGC- -5' |
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| 7563 | 3' | -54.9 | NC_001962.1 | + | 78355 | 0.66 | 0.901106 |
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Target: 5'- ---aUCGUUUCAgGCUGCGGCccAGCGa -3' miRNA: 3'- guugAGCAGGGU-CGACGCCGcuUUGC- -5' |
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| 7563 | 3' | -54.9 | NC_001962.1 | + | 49624 | 0.67 | 0.887677 |
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Target: 5'- aCGAUUUG--CCGGCUGCGGCGccGACa -3' miRNA: 3'- -GUUGAGCagGGUCGACGCCGCu-UUGc -5' |
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| 7563 | 3' | -54.9 | NC_001962.1 | + | 23951 | 0.68 | 0.833614 |
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Target: 5'- aAACUUG-CCCAGCuUGCGGCccuuuGAAAUc -3' miRNA: 3'- gUUGAGCaGGGUCG-ACGCCG-----CUUUGc -5' |
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| 7563 | 3' | -54.9 | NC_001962.1 | + | 81532 | 0.69 | 0.770333 |
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Target: 5'- aCAACgcugccauagCGUCUCAGUaGCGGCGGgaAACGc -3' miRNA: 3'- -GUUGa---------GCAGGGUCGaCGCCGCU--UUGC- -5' |
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| 7563 | 3' | -54.9 | NC_001962.1 | + | 13092 | 1.09 | 0.003251 |
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Target: 5'- aCAACUCGUCCCAGCUGCGGCGAAACGu -3' miRNA: 3'- -GUUGAGCAGGGUCGACGCCGCUUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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