Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7564 | 3' | -52.2 | NC_001962.1 | + | 45028 | 0.67 | 0.96053 |
Target: 5'- --aGAACGAGGcCGUGuuGaGAAACGu -3' miRNA: 3'- caaUUUGCUCC-GCGCggCgCUUUGCc -5' |
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7564 | 3' | -52.2 | NC_001962.1 | + | 102935 | 0.68 | 0.929067 |
Target: 5'- --cGAACGAGGUcUGCCGUGAggagAACGu -3' miRNA: 3'- caaUUUGCUCCGcGCGGCGCU----UUGCc -5' |
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7564 | 3' | -52.2 | NC_001962.1 | + | 6224 | 0.7 | 0.87072 |
Target: 5'- cGUUGGACGAcGGCG-GCgGCagGGAAUGGg -3' miRNA: 3'- -CAAUUUGCU-CCGCgCGgCG--CUUUGCC- -5' |
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7564 | 3' | -52.2 | NC_001962.1 | + | 7076 | 0.7 | 0.863047 |
Target: 5'- -gUGAACGGGGU--GCCGCGccACGGc -3' miRNA: 3'- caAUUUGCUCCGcgCGGCGCuuUGCC- -5' |
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7564 | 3' | -52.2 | NC_001962.1 | + | 21362 | 1.1 | 0.005492 |
Target: 5'- uGUUAAACGAGGCGCGCCGCGAAACGGc -3' miRNA: 3'- -CAAUUUGCUCCGCGCGGCGCUUUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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