Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 7566 | 3' | -59.2 | NC_001962.1 | + | 36381 | 0.66 | 0.759714 |
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Target: 5'- aUGGCcg-GUGuGCUGGUGAGCAACgCa -3' miRNA: 3'- -ACCGcugCGCuCGGCCGUUCGUUGgG- -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 64334 | 0.66 | 0.750226 |
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Target: 5'- aGGUGGUGCG-GuuGGCG-GCGGCgCCa -3' miRNA: 3'- aCCGCUGCGCuCggCCGUuCGUUG-GG- -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 33464 | 0.66 | 0.749271 |
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Target: 5'- cGGCGGCGgauCGGGCaauuugcuGCAAaugaugcGCAGCCCg -3' miRNA: 3'- aCCGCUGC---GCUCGgc------CGUU-------CGUUGGG- -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 67655 | 0.66 | 0.711345 |
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Target: 5'- cGGCGGCGUcGGCgcacUGGCAGGUccugcGCCUg -3' miRNA: 3'- aCCGCUGCGcUCG----GCCGUUCGu----UGGG- -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 67808 | 0.67 | 0.661324 |
|
Target: 5'- aGGCGAcacaCGUGAGCUGGaCAgaacgcuuAGCGgauacgaagaauACCCg -3' miRNA: 3'- aCCGCU----GCGCUCGGCC-GU--------UCGU------------UGGG- -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 126730 | 0.68 | 0.641095 |
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Target: 5'- aGGU-ACGUGcucGgCGGCGAGCAGCCg -3' miRNA: 3'- aCCGcUGCGCu--CgGCCGUUCGUUGGg -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 110263 | 0.69 | 0.548593 |
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Target: 5'- gGGCGugGaCGAuguGCCGGCuuuuaacguuCGGCCCg -3' miRNA: 3'- aCCGCugC-GCU---CGGCCGuuc-------GUUGGG- -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 50872 | 0.69 | 0.530893 |
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Target: 5'- gUGGCGGCggugguugcaucGCGGGCacaGGCGccGGCGGCUg -3' miRNA: 3'- -ACCGCUG------------CGCUCGg--CCGU--UCGUUGGg -5' |
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| 7566 | 3' | -59.2 | NC_001962.1 | + | 60315 | 1.12 | 0.000775 |
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Target: 5'- cUGGCGACGCGAGCCGGCAAGCAACCCa -3' miRNA: 3'- -ACCGCUGCGCUCGGCCGUUCGUUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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