Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7566 | 5' | -50.3 | NC_001962.1 | + | 78103 | 0.66 | 0.991482 |
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Target: 5'- ----gGUUGgCUUCCgccaaaucuGCGUUGGCCa -3' miRNA: 3'- uuuugCGAUgGAAGGau-------UGCAGCCGG- -5' |
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| 7566 | 5' | -50.3 | NC_001962.1 | + | 30446 | 0.66 | 0.987268 |
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Target: 5'- gAGAGCGUuuUACCaagUCCcauguCGUCGGCa -3' miRNA: 3'- -UUUUGCG--AUGGa--AGGauu--GCAGCCGg -5' |
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| 7566 | 5' | -50.3 | NC_001962.1 | + | 91616 | 0.67 | 0.981604 |
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Target: 5'- gGGAACGCcgagGCCUUaUCUaugcaacaAugGUCGGCUg -3' miRNA: 3'- -UUUUGCGa---UGGAA-GGA--------UugCAGCCGG- -5' |
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| 7566 | 5' | -50.3 | NC_001962.1 | + | 32033 | 0.68 | 0.968252 |
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Target: 5'- cGAAGCGCUugCgUCUUAACG-CGaCCg -3' miRNA: 3'- -UUUUGCGAugGaAGGAUUGCaGCcGG- -5' |
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| 7566 | 5' | -50.3 | NC_001962.1 | + | 67671 | 0.7 | 0.91154 |
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Target: 5'- cGAACGCUACUUUgggCggcGGCGUCGGCg -3' miRNA: 3'- uUUUGCGAUGGAAg--Ga--UUGCAGCCGg -5' |
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| 7566 | 5' | -50.3 | NC_001962.1 | + | 60121 | 0.74 | 0.780386 |
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Target: 5'- -cAACGCUauuauugccgcgGCCgcgCCUAACGucacUCGGCCa -3' miRNA: 3'- uuUUGCGA------------UGGaa-GGAUUGC----AGCCGG- -5' |
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| 7566 | 5' | -50.3 | NC_001962.1 | + | 60378 | 0.75 | 0.687695 |
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Target: 5'- gAGAACGUUGCgUUUUUGgcgccgcuaucuGCGUCGGCCa -3' miRNA: 3'- -UUUUGCGAUGgAAGGAU------------UGCAGCCGG- -5' |
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| 7566 | 5' | -50.3 | NC_001962.1 | + | 60279 | 1.11 | 0.00587 |
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Target: 5'- cAAAACGCUACCUUCCUAACGUCGGCCg -3' miRNA: 3'- -UUUUGCGAUGGAAGGAUUGCAGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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