Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 7567 | 3' | -52.5 | NC_001962.1 | + | 11751 | 0.66 | 0.970943 |
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Target: 5'- -cGUCGCAAcauauacauaaaaagGCGCGccGAAGCCGa-- -3' miRNA: 3'- uaCAGCGUU---------------UGUGCuuCUUCGGCggc -5' |
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| 7567 | 3' | -52.5 | NC_001962.1 | + | 104980 | 0.66 | 0.969732 |
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Target: 5'- uUGUUGCAGaacguuucgucGCGCGGcacAGAAaCCGCCa -3' miRNA: 3'- uACAGCGUU-----------UGUGCU---UCUUcGGCGGc -5' |
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| 7567 | 3' | -52.5 | NC_001962.1 | + | 29784 | 0.66 | 0.963131 |
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Target: 5'- cGUGUCGuCGGACACGcacaAAGAAuCCGCa- -3' miRNA: 3'- -UACAGC-GUUUGUGC----UUCUUcGGCGgc -5' |
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| 7567 | 3' | -52.5 | NC_001962.1 | + | 67621 | 0.67 | 0.947061 |
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Target: 5'- gGUGUUGUucucggcgAAGCagGCGGAGGuguAGCCGCCu -3' miRNA: 3'- -UACAGCG--------UUUG--UGCUUCU---UCGGCGGc -5' |
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| 7567 | 3' | -52.5 | NC_001962.1 | + | 465 | 0.67 | 0.932359 |
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Target: 5'- -gGUCGUuuACaAUGggGAAGCUGUCc -3' miRNA: 3'- uaCAGCGuuUG-UGCuuCUUCGGCGGc -5' |
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| 7567 | 3' | -52.5 | NC_001962.1 | + | 74480 | 0.68 | 0.915324 |
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Target: 5'- uUGcCGCAGACGCucAAGAAGCCcaCCGa -3' miRNA: 3'- uACaGCGUUUGUGc-UUCUUCGGc-GGC- -5' |
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| 7567 | 3' | -52.5 | NC_001962.1 | + | 27140 | 0.69 | 0.858762 |
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Target: 5'- -cGUCGCGcguaccggAGCGCGAAcgcgcccGCCGCCGa -3' miRNA: 3'- uaCAGCGU--------UUGUGCUUcuu----CGGCGGC- -5' |
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| 7567 | 3' | -52.5 | NC_001962.1 | + | 82069 | 1.06 | 0.006992 |
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Target: 5'- cAUGUCGCAAACACGAAGAAGCCGCCGc -3' miRNA: 3'- -UACAGCGUUUGUGCUUCUUCGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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