Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
7567 | 5' | -54.3 | NC_001962.1 | + | 98679 | 0.66 | 0.929579 |
Target: 5'- cCCGGCAcGCCggugaucgaUUgGGC-CGAACGCa -3' miRNA: 3'- cGGCCGUuUGGg--------AAgCCGcGUUUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 11349 | 0.66 | 0.924164 |
Target: 5'- uGCCGGaCGAagaaAUCauuagUCGGCGCAcgccAACGUu -3' miRNA: 3'- -CGGCC-GUU----UGGga---AGCCGCGU----UUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 63744 | 0.67 | 0.898726 |
Target: 5'- cGCCGGUuaacggguuGAGCCCgaUGGCGUuuuugucucuCGCa -3' miRNA: 3'- -CGGCCG---------UUUGGGaaGCCGCGuuu-------GCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 82877 | 0.68 | 0.875033 |
Target: 5'- aGUCGuGCAAGCCCgcaaacaaaauucaaUguuUCGaGUGCAAAUGCa -3' miRNA: 3'- -CGGC-CGUUUGGG---------------A---AGC-CGCGUUUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 109410 | 0.68 | 0.86451 |
Target: 5'- cGCUGGCAGAUUuggUUUUGGCGCuAugGg -3' miRNA: 3'- -CGGCCGUUUGG---GAAGCCGCGuUugCg -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 27169 | 0.68 | 0.851965 |
Target: 5'- aGCCGGacaAGgcugucuugcuguguGCCCgUCGcgcguaccggaGCGCGAACGCg -3' miRNA: 3'- -CGGCCg--UU---------------UGGGaAGC-----------CGCGUUUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 61041 | 0.68 | 0.848745 |
Target: 5'- cGCCGGCGAAUCUaUUUGGUacaaCAAAUGUg -3' miRNA: 3'- -CGGCCGUUUGGG-AAGCCGc---GUUUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 13814 | 0.69 | 0.823588 |
Target: 5'- gGCCuGCGcuAUCUUcuaUCGGCGUAAACGUg -3' miRNA: 3'- -CGGcCGUu-UGGGA---AGCCGCGUUUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 83471 | 0.69 | 0.79681 |
Target: 5'- cGCUGGaCGAuACCC-UCGGCgGCGAuUGCg -3' miRNA: 3'- -CGGCC-GUU-UGGGaAGCCG-CGUUuGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 73452 | 0.7 | 0.758993 |
Target: 5'- cCCGGCGAACCgggUUCGG-GCAAGuCGUc -3' miRNA: 3'- cGGCCGUUUGGg--AAGCCgCGUUU-GCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 44071 | 0.71 | 0.698974 |
Target: 5'- -aUGGCAAACCaugUC-GCGCGAGCGUc -3' miRNA: 3'- cgGCCGUUUGGga-AGcCGCGUUUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 71407 | 0.75 | 0.503885 |
Target: 5'- cGUCGGCG--UCCgugUCGGCGCuAACGUa -3' miRNA: 3'- -CGGCCGUuuGGGa--AGCCGCGuUUGCG- -5' |
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7567 | 5' | -54.3 | NC_001962.1 | + | 82034 | 1.15 | 0.001459 |
Target: 5'- cGCCGGCAAACCCUUCGGCGCAAACGCa -3' miRNA: 3'- -CGGCCGUUUGGGAAGCCGCGUUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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